Version 3.0 free energy parameters for RNA folding at 37°

Stacking energies
Terminal mismatch stacking energies (hairpin loops)
Terminal mismatch stacking energies (interior loops)
1 × 1 interior loop energies
1 × 2 interior loop energies
2 × 2 interior loop energies
Single base stacking energies
Loop destabilizing energies
Tetra-loops
Tri-loops
Miscellaneous energies

Stacking energies Data Arangement: Y ------------------ (X) A C G U ------------------ 5' ==> 3' AX AY 3' <== 5' (A) . . . . (C) . . . . (G) . . . . (U) -0.70 -0.10 -0.70 -0.10 -------------------------------- STACKING ENERGIES : Y Y Y Y ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' AX AX AX AX AY CY GY UY 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' . . . . . . . . . . . . . . . -0.90 . . . . . . . . . . . . . . -2.20 . . . . . . . . . . . . . . -2.10 . -0.60 . . . . . . . . . . . . -1.10 . -1.40 . Y Y Y Y ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' CX CX CX CX AY CY GY UY 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' . . . . . . . . . . . -2.10 . . . . . . . . . . . . . . -3.30 . . . . . . . . . . . . . . -2.40 . -1.40 . . . . . . . . . . . . -2.10 . -2.10 . . . . . Y Y Y Y ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' GX GX GX GX AY CY GY UY 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' . . . . . . . -2.40 . . . . . . . -1.30 . . . . . . -3.40 . . . . . . . -2.50 . . . . . . -3.30 . -1.50 . . . . . -2.10 . -0.50 . . . . -2.20 . -2.50 . . . . . -1.40 . 1.30 . Y Y Y Y ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' UX UX UX UX AY CY GY UY 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' . . . -1.30 . . . . . . . -1.00 . . . . . . -2.40 . . . . . . . -1.50 . . . . . . -2.10 . -1.00 . . . . . -1.40 . 0.30 . . . . -0.90 . -1.30 . . . . . -0.60 . -0.50 . . . . .

Terminal mismatch stacking energies (hairpin loops) Data Arangement: Y ------------------ (X) A C G U ------------------ 5' ==> 3' AX AY 3' <== 5' (A) . . . . (C) . . . . (G) . . . . (U) -0.70 -0.10 -0.70 -0.10 -------------------------------- STACKING ENERGIES : TERMINAL MISMATCHES AND BASE-PAIRS Y Y Y Y ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' AX AX AX AX AY CY GY UY 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' . . . . . . . . . . . . -0.30 -0.50 -0.30 -0.30 . . . . . . . . . . . . -0.10 -0.20 -1.50 -0.20 . . . . . . . . . . . . -1.10 -1.20 -0.20 0.20 . . . . . . . . . . . . -0.30 -0.30 -0.60 -1.10 Y Y Y Y ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' CX CX CX CX AY CY GY UY 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' . . . . . . . . -1.50 -1.50 -1.40 -1.80 . . . . . . . . . . . . -1.00 -0.90 -2.90 -0.80 . . . . . . . . . . . . -2.20 -2.00 -1.60 -1.10 . . . . . . . . . . . . -1.70 -1.40 -1.80 -2.00 . . . . Y Y Y Y ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' GX GX GX GX AY CY GY UY 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' . . . . -1.10 -1.50 -1.30 -2.10 . . . . 0.20 -0.50 -0.30 -0.30 . . . . -1.10 -0.70 -2.40 -0.50 . . . . -0.10 -0.20 -1.50 -0.20 . . . . -2.40 -2.90 -1.40 -1.20 . . . . -0.90 -1.10 -0.30 0.00 . . . . -1.90 -1.00 -2.20 -1.50 . . . . -0.30 -0.30 -0.40 -1.10 Y Y Y Y ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' UX UX UX UX AY CY GY UY 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' -0.50 -0.30 -0.60 -0.50 . . . . -0.50 -0.30 -0.60 -0.50 . . . . -0.20 -0.10 -1.20 -0.00 . . . . -0.20 -0.10 -1.70 0.00 . . . . -1.40 -1.20 -0.70 -0.20 . . . . -0.80 -1.20 -0.30 -0.70 . . . . -0.30 -0.10 -0.50 -0.80 . . . . -0.60 -0.10 -0.60 -0.80 . . . .

Terminal mismatch stacking energies (interior loops) Data Arangement: Y ------------------ (X) A C G U ------------------ 5' ==> 3' AX AY 3' <== 5' (A) . . . . (C) . . . . (G) . . . . (U) -0.70 -0.10 -0.70 -0.10 -------------------------------- STACKING ENERGIES : TERMINAL MISMATCHES AND BASE-PAIRS Y Y Y Y ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' AX AX AX AX AY CY GY UY 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' . . . . . . . . . . . . 0.70 0.70 -0.40 0.70 . . . . . . . . . . . . 0.70 0.70 0.70 0.70 . . . . . . . . . . . . -0.40 0.70 0.70 0.70 . . . . . . . . . . . . 0.70 0.70 0.70 0.00 Y Y Y Y ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' CX CX CX CX AY CY GY UY 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' . . . . . . . . -0.00 -0.00 -1.10 -0.00 . . . . . . . . . . . . -0.00 -0.00 -0.00 -0.00 . . . . . . . . . . . . -1.10 -0.00 -0.00 -0.00 . . . . . . . . . . . . -0.00 -0.00 -0.00 -0.70 . . . . Y Y Y Y ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' GX GX GX GX AY CY GY UY 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' . . . . -0.00 -0.00 -1.10 -0.00 . . . . 0.70 0.70 -0.40 0.70 . . . . -0.00 -0.00 -0.00 -0.00 . . . . 0.70 0.70 0.70 0.70 . . . . -1.10 -0.00 -0.00 -0.00 . . . . -0.40 0.70 0.70 0.70 . . . . -0.00 -0.00 -0.00 -0.70 . . . . 0.70 0.70 0.70 0.00 Y Y Y Y ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' UX UX UX UX AY CY GY UY 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 0.70 0.70 -0.40 0.70 . . . . 0.70 0.70 -0.40 0.70 . . . . 0.70 0.70 0.70 0.70 . . . . 0.70 0.70 0.70 0.70 . . . . -0.40 0.70 0.70 0.70 . . . . -0.40 0.70 0.70 0.70 . . . . 0.70 0.70 0.70 0.00 . . . . 0.70 0.70 0.70 0.00 . . . .

Single mismatch energies (1 × 1 interior loops) Data table for symetric interior loops of size 2 Free energies at 37 degrees for RNA Data Arrangement: Y ------------------ (X) A C G U ------------------ 5' --> 3' X A A U U Y 3' <-- 5' (A) . . . . (C) . . . . (G) . . . . (U) . . . . Y Y Y Y Y Y ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' X X X X X X A A A C A G A U A G A U U U U G U C U A U U U G Y Y Y Y Y Y 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 1.70 1.70 1.70 1.70 1.10 1.10 1.10 1.10 1.10 1.10 1.10 1.10 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.10 1.10 1.10 1.10 1.10 1.10 1.10 1.10 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 -0.40 1.70 1.10 1.10 -1.00 1.10 1.10 1.10 -1.00 1.10 1.70 1.70 -0.40 1.70 1.70 1.70 -0.40 1.70 1.70 1.70 -0.40 1.70 1.70 1.70 1.70 1.50 1.10 1.10 1.10 1.00 1.10 1.10 1.10 1.10 1.70 1.70 1.70 1.20 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 Y Y Y Y Y Y ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' X X X X X X C A C C C G C U C G C U G U G G G C G A G U G G Y Y Y Y Y Y 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 1.10 1.10 1.10 1.10 0.40 -0.40 0.40 0.40 1.10 0.40 0.40 0.40 1.10 1.10 1.10 1.10 1.10 1.10 1.10 1.10 1.10 1.10 1.10 1.10 1.10 1.10 1.10 1.10 0.30 0.50 0.40 0.50 0.40 0.40 0.40 0.40 1.10 1.10 1.10 1.10 1.10 1.10 1.10 1.10 1.10 1.10 1.10 1.10 1.10 1.10 -1.00 1.10 -0.10 0.40 -1.70 0.40 0.40 0.40 -1.40 0.40 1.10 1.10 -1.00 1.10 1.10 1.10 -1.00 1.10 1.10 1.10 -1.00 1.10 1.10 1.10 1.10 1.10 0.40 0.00 0.40 -0.30 0.40 0.40 0.40 0.40 1.10 1.10 1.10 1.10 1.10 1.10 1.10 1.10 1.10 1.10 1.10 1.10 Y Y Y Y Y Y ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' X X X X X X G A G C G G G U G G G U C U C G C C C A C U C G Y Y Y Y Y Y 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 1.10 1.10 1.10 1.10 0.80 0.40 0.40 0.40 0.40 0.30 -0.10 0.40 1.10 1.10 1.10 1.10 1.10 1.10 1.10 1.10 1.10 1.10 1.10 1.10 1.10 1.10 1.10 1.10 0.40 0.40 0.40 0.40 -0.40 0.50 0.40 0.00 1.10 1.10 1.10 1.10 1.10 1.10 1.10 1.10 1.10 1.10 1.10 1.10 1.10 1.10 -1.00 1.10 0.40 0.40 -2.10 0.40 0.40 0.40 -1.70 0.40 1.10 1.10 -1.00 1.10 1.10 1.10 -1.00 1.10 1.10 1.10 -1.00 1.10 1.10 1.10 1.10 1.10 0.40 0.40 0.40 -0.70 0.40 0.50 0.40 -0.30 1.10 1.10 1.10 1.00 1.10 1.10 1.10 1.10 1.10 1.10 1.10 1.10 Y Y Y Y Y Y ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' X X X X X X U A U C U G U U U G U U A U A G A C A A A U A G Y Y Y Y Y Y 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 1.70 1.70 1.70 1.70 1.10 1.10 1.10 1.10 1.10 1.10 1.10 1.10 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.10 1.10 1.10 1.10 1.10 1.10 1.10 1.10 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 -0.40 1.70 1.10 1.10 -1.00 1.10 1.10 1.10 -1.00 1.10 1.70 1.70 -0.40 1.70 1.70 1.70 -0.40 1.70 1.70 1.70 -0.40 1.70 1.70 1.70 1.70 1.80 1.10 1.10 1.10 1.10 1.10 1.10 1.10 1.10 1.70 1.70 1.70 1.50 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 Y Y Y Y Y Y ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' X X X X X X G A G C G G G U G G G U U U U G U C U A U U U G Y Y Y Y Y Y 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 1.70 1.70 1.70 1.70 1.10 1.10 1.10 1.10 1.10 1.10 1.10 1.10 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.10 1.10 1.10 1.10 1.10 1.10 1.10 1.10 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 -0.40 1.70 1.10 1.10 -1.00 1.10 1.10 1.10 -1.00 1.10 1.70 1.70 -0.40 1.70 1.70 1.70 -0.40 1.70 1.70 1.70 -0.40 1.70 1.70 1.70 1.70 1.70 1.10 1.10 1.10 1.10 1.10 1.10 1.10 1.10 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 Y Y Y Y Y Y ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' X X X X X X U A U C U G U U U G U U G U G G G C G A G U G G Y Y Y Y Y Y 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 1.70 1.70 1.70 1.70 1.10 1.10 1.10 1.10 1.10 1.10 1.10 1.10 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.10 1.10 1.10 1.10 1.10 1.10 1.10 1.10 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 -0.40 1.70 1.10 1.10 -1.00 1.10 1.10 1.10 -1.00 1.10 1.70 1.70 -0.40 1.70 1.70 1.70 -0.40 1.70 1.70 1.70 -0.40 1.70 1.70 1.70 1.70 1.70 1.10 1.10 1.10 1.10 1.10 1.10 1.10 1.10 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70 1.70

1 × 2 interior loop energies) Data tables for asymmetric interior loops of size 3 Free energies at 37 degrees for RNA Data arrangement: Y ------------------ (X) A C G U ------------------ 5' --> 3' X A A U U YA 3' <-- 5' (A) . . . . (C) . . . . (G) . . . . (U) . . . . Y Y Y Y Y Y ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' X X X X X X A A A C A G A U A G A U U U U G U C U A U U U G YA YA YA YA YA YA 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3.90 3.70 3.10 5.50 3.20 3.00 2.40 4.80 3.20 3.00 2.40 4.80 3.90 3.70 3.10 5.50 3.90 3.70 3.10 5.50 3.90 3.70 3.10 5.50 3.80 3.70 5.50 3.70 3.10 3.00 4.80 3.00 3.10 3.00 4.80 3.00 3.80 3.70 5.50 3.70 3.80 3.70 5.50 3.70 3.80 3.70 5.50 3.70 3.20 5.50 2.30 5.50 2.50 4.80 1.60 4.80 2.50 4.80 1.60 4.80 3.20 5.50 2.30 5.50 3.20 5.50 2.30 5.50 3.20 5.50 2.30 5.50 5.50 5.50 5.50 5.50 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 Y Y Y Y Y Y ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' X X X X X X A A A C A G A U A G A U U U U G U C U A U U U G YC YC YC YC YC YC 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3.60 3.20 3.10 5.50 2.90 2.50 2.40 4.80 2.90 2.50 2.40 4.80 3.60 3.20 3.10 5.50 3.60 3.20 3.10 5.50 3.60 3.20 3.10 5.50 3.70 4.00 5.50 3.70 3.00 3.30 4.80 3.00 3.00 3.30 4.80 3.00 3.70 4.00 5.50 3.70 3.70 4.00 5.50 3.70 3.70 4.00 5.50 3.70 5.50 5.50 5.50 5.50 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 3.70 5.50 2.80 4.80 3.00 4.80 2.10 4.80 3.00 4.80 2.10 5.50 3.70 5.50 2.80 5.50 3.70 5.50 2.80 5.50 3.70 5.50 2.80 Y Y Y Y Y Y ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' X X X X X X A A A C A G A U A G A U U U U G U C U A U U U G YG YG YG YG YG YG 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 2.50 2.10 1.90 5.50 1.80 1.40 1.20 4.80 1.80 1.40 1.20 4.80 2.50 2.10 1.90 5.50 2.50 2.10 1.90 5.50 2.50 2.10 1.90 5.50 5.50 5.50 5.50 5.50 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 2.30 5.50 3.70 5.50 1.60 4.80 3.00 4.80 1.60 4.80 3.00 4.80 2.30 5.50 3.70 5.50 2.30 5.50 3.70 5.50 2.30 5.50 3.70 5.50 5.50 5.50 5.50 5.50 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 Y Y Y Y Y Y ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' X X X X X X A A A C A G A U A G A U U U U G U C U A U U U G YU YU YU YU YU YU 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 5.50 5.50 5.50 5.50 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 4.00 3.40 5.50 3.70 3.30 2.70 4.80 3.00 3.30 2.70 4.80 3.00 4.00 3.40 5.50 3.70 4.00 3.40 5.50 3.70 4.00 3.40 5.50 3.70 5.50 5.50 5.50 5.50 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 3.20 5.50 2.70 4.80 2.50 4.80 2.00 4.80 2.50 4.80 2.00 5.50 3.20 5.50 2.70 5.50 3.20 5.50 2.70 5.50 3.20 5.50 2.70 Y Y Y Y Y Y ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' X X X X X X C A C C C G C U C G C U G U G G G C G A G U G G YA YA YA YA YA YA 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3.20 3.00 2.40 4.80 2.30 2.20 1.10 4.00 2.40 2.20 1.60 4.00 3.20 3.00 2.40 4.80 3.20 3.00 2.40 4.80 3.20 3.00 2.40 4.80 3.10 3.00 4.80 3.00 2.30 2.20 4.00 2.20 2.30 2.20 4.00 2.20 3.10 3.00 4.80 3.00 3.10 3.00 4.80 3.00 3.10 3.00 4.80 3.00 2.50 4.80 1.60 4.80 1.70 4.00 0.80 4.00 1.70 4.00 0.80 4.00 2.50 4.80 1.60 4.80 2.50 4.80 1.60 4.80 2.50 4.80 1.60 4.80 4.80 4.80 4.80 4.80 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 Y Y Y Y Y Y ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' X X X X X X C A C C C G C U C G C U G U G G G C G A G U G G YC YC YC YC YC YC 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 2.90 2.50 2.40 4.80 2.10 1.70 1.60 4.00 2.10 1.70 1.60 4.00 2.90 2.50 2.40 4.80 2.90 2.50 2.40 4.80 2.90 2.50 2.40 4.80 3.00 3.30 4.80 3.00 2.20 2.50 4.00 2.20 2.20 2.50 4.00 2.20 3.00 3.30 4.80 3.00 3.00 3.30 4.80 3.00 3.00 3.30 4.80 3.00 4.80 4.80 4.80 4.80 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 3.00 4.80 2.10 4.00 2.20 4.00 1.50 4.00 2.20 4.00 1.30 4.80 3.00 4.80 2.10 4.80 3.00 4.80 2.10 4.80 3.00 4.80 2.10 Y Y Y Y Y Y ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' X X X X X X C A C C C G C U C G C U G U G G G C G A G U G G YG YG YG YG YG YG 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 1.80 1.40 1.20 4.80 0.80 0.60 0.40 4.00 1.00 0.60 0.40 4.00 1.80 1.40 1.20 4.80 1.80 1.40 1.20 4.80 1.80 1.40 1.20 4.80 4.80 4.80 4.80 4.80 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 1.60 4.80 3.00 4.80 0.80 4.00 2.20 4.00 0.80 4.00 2.20 4.00 1.60 4.80 3.00 4.80 1.60 4.80 3.00 4.80 1.60 4.80 3.00 4.80 4.80 4.80 4.80 4.80 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 Y Y Y Y Y Y ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' X X X X X X C A C C C G C U C G C U G U G G G C G A G U G G YU YU YU YU YU YU 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 4.80 4.80 4.80 4.80 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 3.30 2.70 4.80 3.00 2.50 1.90 4.00 2.20 2.50 1.90 4.00 2.20 3.30 2.70 4.80 3.00 3.30 2.70 4.80 3.00 3.30 2.70 4.80 3.00 4.80 4.80 4.80 4.80 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 2.50 4.80 2.00 4.00 1.70 4.00 1.20 4.00 1.70 4.00 1.20 4.80 2.50 4.80 2.00 4.80 2.50 4.80 2.00 4.80 2.50 4.80 2.00 Y Y Y Y Y Y ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' X X X X X X G A G C G G G U G G G U C U C G C C C A C U C G YA YA YA YA YA YA 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3.20 3.00 2.40 4.80 2.40 2.20 1.60 4.00 2.50 2.20 2.10 4.00 3.20 3.00 2.40 4.80 3.20 3.00 2.40 4.80 3.20 3.00 2.40 4.80 3.10 3.00 4.80 3.00 2.30 2.20 4.00 2.20 2.30 2.20 4.00 2.20 3.10 3.00 4.80 3.00 3.10 3.00 4.80 3.00 3.10 3.00 4.80 3.00 2.50 4.80 1.60 4.80 1.70 4.00 0.80 4.00 1.70 4.00 0.80 4.00 2.50 4.80 1.60 4.80 2.50 4.80 1.60 4.80 2.50 4.80 1.60 4.80 4.80 4.80 4.80 4.80 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 Y Y Y Y Y Y ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' X X X X X X G A G C G G G U G G G U C U C G C C C A C U C G YC YC YC YC YC YC 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 2.90 2.50 2.40 4.80 2.10 1.70 1.60 4.00 2.10 1.70 1.60 4.00 2.90 2.50 2.40 4.80 2.90 2.50 2.40 4.80 2.90 2.50 2.40 4.80 3.00 3.30 4.80 3.00 2.20 2.50 4.00 2.20 2.20 2.50 4.00 2.20 3.00 3.30 4.80 3.00 3.00 3.30 4.80 3.00 3.00 3.30 4.80 3.00 4.80 4.80 4.80 4.80 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 3.00 4.80 2.10 4.00 2.20 4.00 1.30 4.00 2.20 4.00 1.20 4.80 3.00 4.80 2.10 4.80 3.00 4.80 2.10 4.80 3.00 4.80 2.10 Y Y Y Y Y Y ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' X X X X X X G A G C G G G U G G G U C U C G C C C A C U C G YG YG YG YG YG YG 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 1.80 1.40 1.20 4.80 1.00 0.60 0.40 4.00 1.20 0.60 0.40 4.00 1.80 1.40 1.20 4.80 1.80 1.40 1.20 4.80 1.80 1.40 1.20 4.80 4.80 4.80 4.80 4.80 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 1.60 4.80 3.00 4.80 0.80 4.00 2.20 4.00 0.80 4.00 2.20 4.00 1.60 4.80 3.00 4.80 1.60 4.80 3.00 4.80 1.60 4.80 3.00 4.80 4.80 4.80 4.80 4.80 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 Y Y Y Y Y Y ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' X X X X X X G A G C G G G U G G G U C U C G C C C A C U C G YU YU YU YU YU YU 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 4.80 4.80 4.80 4.80 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 3.30 2.70 4.80 3.00 2.50 1.90 4.00 2.20 2.50 1.90 4.00 2.20 3.30 2.70 4.80 3.00 3.30 2.70 4.80 3.00 3.30 2.70 4.80 3.00 4.80 4.80 4.80 4.80 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 2.50 4.80 2.00 4.00 1.70 4.00 1.20 4.00 1.70 4.00 1.20 4.80 2.50 4.80 2.00 4.80 2.50 4.80 2.00 4.80 2.50 4.80 2.00 Y Y Y Y Y Y ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' X X X X X X U A U C U G U U U G U U A U A G A C A A A U A G YA YA YA YA YA YA 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3.90 3.70 3.10 5.50 3.20 3.00 2.40 4.80 3.20 3.00 2.40 4.80 3.90 3.70 3.10 5.50 3.90 3.70 3.10 5.50 3.90 3.70 3.10 5.50 3.80 3.70 5.50 3.70 3.10 3.00 4.80 3.00 3.10 3.00 4.80 3.00 3.80 3.70 5.50 3.70 3.80 3.70 5.50 3.70 3.80 3.70 5.50 3.70 3.20 5.50 2.30 5.50 2.50 4.80 1.60 4.80 2.50 4.80 1.60 4.80 3.20 5.50 2.30 5.50 3.20 5.50 2.30 5.50 3.20 5.50 2.30 5.50 5.50 5.50 5.50 5.50 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 Y Y Y Y Y Y ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' X X X X X X U A U C U G U U U G U U A U A G A C A A A U A G YC YC YC YC YC YC 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3.60 3.20 3.10 5.50 2.90 2.50 2.40 4.80 2.90 2.50 2.40 4.80 3.60 3.20 3.10 5.50 3.60 3.20 3.10 5.50 3.60 3.20 3.10 5.50 3.70 4.00 5.50 3.70 3.00 3.30 4.80 3.00 3.00 3.30 4.80 3.00 3.70 4.00 5.50 3.70 3.70 4.00 5.50 3.70 3.70 4.00 5.50 3.70 5.50 5.50 5.50 5.50 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 3.70 5.50 2.80 4.80 3.00 4.80 2.10 4.80 3.00 4.80 2.10 5.50 3.70 5.50 2.80 5.50 3.70 5.50 2.80 5.50 3.70 5.50 2.80 Y Y Y Y Y Y ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' X X X X X X U A U C U G U U U G U U A U A G A C A A A U A G YG YG YG YG YG YG 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 2.50 2.10 1.90 5.50 1.80 1.40 1.20 4.80 1.80 1.40 1.20 4.80 2.50 2.10 1.90 5.50 2.50 2.10 1.90 5.50 2.50 2.10 1.90 5.50 5.50 5.50 5.50 5.50 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 2.30 5.50 3.70 5.50 1.60 4.80 3.00 4.80 1.60 4.80 3.00 4.80 2.30 5.50 3.70 5.50 2.30 5.50 3.70 5.50 2.30 5.50 3.70 5.50 5.50 5.50 5.50 5.50 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 Y Y Y Y Y Y ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' X X X X X X U A U C U G U U U G U U A U A G A C A A A U A G YU YU YU YU YU YU 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 5.50 5.50 5.50 5.50 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 4.00 3.40 5.50 3.70 3.30 2.70 4.80 3.00 3.30 2.70 4.80 3.00 4.00 3.40 5.50 3.70 4.00 3.40 5.50 3.70 4.00 3.40 5.50 3.70 5.50 5.50 5.50 5.50 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 3.20 5.50 2.70 4.80 2.50 4.80 2.00 4.80 2.50 4.80 2.00 5.50 3.20 5.50 2.70 5.50 3.20 5.50 2.70 5.50 3.20 5.50 2.70 Y Y Y Y Y Y ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' X X X X X X G A G C G G G U G G G U U U U G U C U A U U U G YA YA YA YA YA YA 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3.90 3.70 3.10 5.50 3.20 3.00 2.40 4.80 3.20 3.00 2.40 4.80 3.90 3.70 3.10 5.50 3.90 3.70 3.10 5.50 3.90 3.70 3.10 5.50 3.80 3.70 5.50 3.70 3.10 3.00 4.80 3.00 3.10 3.00 4.80 3.00 3.80 3.70 5.50 3.70 3.80 3.70 5.50 3.70 3.80 3.70 5.50 3.70 3.20 5.50 2.30 5.50 2.50 4.80 1.60 4.80 2.50 4.80 1.60 4.80 3.20 5.50 2.30 5.50 3.20 5.50 2.30 5.50 3.20 5.50 2.30 5.50 5.50 5.50 5.50 5.50 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 Y Y Y Y Y Y ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' X X X X X X G A G C G G G U G G G U U U U G U C U A U U U G YC YC YC YC YC YC 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3.60 3.20 3.10 5.50 2.90 2.50 2.40 4.80 2.90 2.50 2.40 4.80 3.60 3.20 3.10 5.50 3.60 3.20 3.10 5.50 3.60 3.20 3.10 5.50 3.70 4.00 5.50 3.70 3.00 3.30 4.80 3.00 3.00 3.30 4.80 3.00 3.70 4.00 5.50 3.70 3.70 4.00 5.50 3.70 3.70 4.00 5.50 3.70 5.50 5.50 5.50 5.50 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 3.70 5.50 2.80 4.80 3.00 4.80 2.10 4.80 3.00 4.80 2.10 5.50 3.70 5.50 2.80 5.50 3.70 5.50 2.80 5.50 3.70 5.50 2.80 Y Y Y Y Y Y ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' X X X X X X G A G C G G G U G G G U U U U G U C U A U U U G YG YG YG YG YG YG 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 2.50 2.10 1.90 5.50 1.80 1.40 1.20 4.80 1.80 1.40 1.20 4.80 2.50 2.10 1.90 5.50 2.50 2.10 1.90 5.50 2.50 2.10 1.90 5.50 5.50 5.50 5.50 5.50 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 2.30 5.50 3.70 5.50 1.60 4.80 3.00 4.80 1.60 4.80 3.00 4.80 2.30 5.50 3.70 5.50 2.30 5.50 3.70 5.50 2.30 5.50 3.70 5.50 5.50 5.50 5.50 5.50 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 Y Y Y Y Y Y ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' X X X X X X G A G C G G G U G G G U U U U G U C U A U U U G YU YU YU YU YU YU 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 5.50 5.50 5.50 5.50 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 4.00 3.40 5.50 3.70 3.30 2.70 4.80 3.00 3.30 2.70 4.80 3.00 4.00 3.40 5.50 3.70 4.00 3.40 5.50 3.70 4.00 3.40 5.50 3.70 5.50 5.50 5.50 5.50 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 3.20 5.50 2.70 4.80 2.50 4.80 2.00 4.80 2.50 4.80 2.00 5.50 3.20 5.50 2.70 5.50 3.20 5.50 2.70 5.50 3.20 5.50 2.70 Y Y Y Y Y Y ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' X X X X X X U A U C U G U U U G U U G U G G G C G A G U G G YA YA YA YA YA YA 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3.90 3.70 3.10 5.50 3.20 3.00 2.40 4.80 3.20 3.00 2.40 4.80 3.90 3.70 3.10 5.50 3.90 3.70 3.10 5.50 3.90 3.70 3.10 5.50 3.80 3.70 5.50 3.70 3.10 3.00 4.80 3.00 3.10 3.00 4.80 3.00 3.80 3.70 5.50 3.70 3.80 3.70 5.50 3.70 3.80 3.70 5.50 3.70 3.20 5.50 2.30 5.50 2.50 4.80 1.60 4.80 2.50 4.80 1.60 4.80 3.20 5.50 2.30 5.50 3.20 5.50 2.30 5.50 3.20 5.50 2.30 5.50 5.50 5.50 5.50 5.50 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 Y Y Y Y Y Y ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' X X X X X X U A U C U G U U U G U U G U G G G C G A G U G G YC YC YC YC YC YC 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3.60 3.20 3.10 5.50 2.90 2.50 2.40 4.80 2.90 2.50 2.40 4.80 3.60 3.20 3.10 5.50 3.60 3.20 3.10 5.50 3.60 3.20 3.10 5.50 3.70 4.00 5.50 3.70 3.00 3.30 4.80 3.00 3.00 3.30 4.80 3.00 3.70 4.00 5.50 3.70 3.70 4.00 5.50 3.70 3.70 4.00 5.50 3.70 5.50 5.50 5.50 5.50 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 3.70 5.50 2.80 4.80 3.00 4.80 2.10 4.80 3.00 4.80 2.10 5.50 3.70 5.50 2.80 5.50 3.70 5.50 2.80 5.50 3.70 5.50 2.80 Y Y Y Y Y Y ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' X X X X X X U A U C U G U U U G U U G U G G G C G A G U G G YG YG YG YG YG YG 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 2.50 2.10 1.90 5.50 1.80 1.40 1.20 4.80 1.80 1.40 1.20 4.80 2.50 2.10 1.90 5.50 2.50 2.10 1.90 5.50 2.50 2.10 1.90 5.50 5.50 5.50 5.50 5.50 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 2.30 5.50 3.70 5.50 1.60 4.80 3.00 4.80 1.60 4.80 3.00 4.80 2.30 5.50 3.70 5.50 2.30 5.50 3.70 5.50 2.30 5.50 3.70 5.50 5.50 5.50 5.50 5.50 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 Y Y Y Y Y Y ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' X X X X X X U A U C U G U U U G U U G U G G G C G A G U G G YU YU YU YU YU YU 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' 5.50 5.50 5.50 5.50 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 4.00 3.40 5.50 3.70 3.30 2.70 4.80 3.00 3.30 2.70 4.80 3.00 4.00 3.40 5.50 3.70 4.00 3.40 5.50 3.70 4.00 3.40 5.50 3.70 5.50 5.50 5.50 5.50 4.80 4.80 4.80 4.80 4.80 4.80 4.80 4.80 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 5.50 3.20 5.50 2.70 4.80 2.50 4.80 2.00 4.80 2.50 4.80 2.00 5.50 3.20 5.50 2.70 5.50 3.20 5.50 2.70 5.50 3.20 5.50 2.70

Tandem mismatch energies (2 × 2 interior loops) Data tables for symetric interior loops of size 4 Free energies at 37 degrees for RNA Data arrangement: Y ---------------------------------------------------------------- (X) A A A A C C C C G G G G U U U U A C G U A C G U A C G U A C G U ---------------------------------------------------------------- 5' ------> 3' A \/ \_/ A U /\ | U 3' <------ 5' (AA) . . . . . . . . . . . . . . . . (AC) . . . . . . . . . . . . . . . . (AG) . . . . . . . . . . . . . . . . (AU) . . . . . . . . . . . . . . . . (CA) . . . . . . . . . . . . . . . . (CC) . . . . . . . . . . . . . . . . (CG) . . . . . . . . . . . . . . . . (CU) . . . . . . . . . . . . . . . . (GA) . . . . . . . . . . . . . . . . (GC) . . . . . . . . . . . . . . . . (GG) . . . . . . . . . . . . . . . . (GU) . . . . . . . . . . . . . . . . (UA) . . . . . . . . . . . . . . . . (UC) . . . . . . . . . . . . . . . . (UG) . . . . . . . . . . . . . . . . (UU) . . . . . . . . . . . . . . . . Y --------------------------------------------------------------------------------------------- A A A A C C C C G G G G U U U U A C G U A C G U A C G U A C G U --------------------------------------------------------------------------------------------- 5' ------> 3' A \/ \_/ A U /\ | U 3' <------ 5' 2.80 2.30 1.70 2.00 2.80 3.40 2.00 3.40 1.70 2.00 2.10 1.00 2.00 3.10 2.20 2.90 2.60 2.20 1.60 2.00 2.60 2.60 2.00 2.60 1.60 2.00 2.00 -0.20 2.00 2.30 1.10 1.80 1.50 1.10 0.50 2.00 1.50 2.50 2.00 2.50 0.50 2.00 0.90 0.50 2.00 2.20 1.80 2.50 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.50 2.10 1.50 2.00 2.50 2.50 2.00 2.50 1.50 2.00 1.90 -0.30 2.00 2.20 1.00 1.70 3.10 2.00 2.40 2.00 2.50 2.50 2.00 2.50 2.40 2.00 2.40 0.70 2.00 2.20 1.90 1.60 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 3.10 2.00 2.40 2.00 2.50 2.50 2.00 2.50 2.40 2.00 2.40 0.70 2.00 2.20 1.90 1.60 1.50 1.10 0.50 2.00 1.50 2.50 2.00 2.50 0.50 2.00 0.90 0.50 2.00 2.20 1.80 2.50 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.10 1.60 1.00 2.00 2.10 2.70 2.00 2.70 1.00 2.00 1.40 0.30 2.00 2.40 1.50 2.20 2.30 0.90 2.10 2.00 1.30 2.30 2.00 2.30 2.10 2.00 1.70 -1.50 2.00 2.00 -0.20 2.30 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 3.10 2.00 2.40 2.00 2.50 2.50 2.00 2.50 2.40 2.00 2.40 0.70 2.00 2.20 1.90 1.60 1.30 -0.10 1.10 2.00 0.30 1.30 2.00 1.30 1.10 2.00 0.70 -2.50 2.00 1.00 -1.20 1.30 2.70 1.20 2.40 2.00 1.70 1.70 2.00 1.70 2.40 2.00 2.00 0.70 2.00 1.40 1.90 0.80 Y --------------------------------------------------------------------------------------------- A A A A C C C C G G G G U U U U A C G U A C G U A C G U A C G U --------------------------------------------------------------------------------------------- 5' ------> 3' A \/ \_/ C U /\ | G 3' <------ 5' 2.10 1.90 0.10 2.00 1.80 2.50 2.00 1.50 0.70 2.00 1.80 0.00 2.00 2.50 0.40 2.10 2.00 1.70 0.00 2.00 1.70 1.70 2.00 0.70 0.60 2.00 1.60 -1.20 2.00 1.80 -0.80 1.00 0.90 0.60 -1.10 2.00 0.60 1.60 2.00 0.70 -0.50 2.00 0.50 -0.50 2.00 1.70 -0.10 1.70 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 1.90 1.60 -0.10 2.00 1.60 1.60 2.00 0.60 0.50 2.00 1.50 -1.30 2.00 1.70 -0.90 0.90 2.40 1.60 0.80 2.00 1.50 1.60 2.00 0.60 1.40 2.00 2.10 -0.30 2.00 1.60 0.10 0.80 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.40 1.60 0.80 2.00 1.50 1.60 2.00 0.60 1.40 2.00 2.10 -0.30 2.00 1.60 0.10 0.80 0.90 0.60 -1.10 2.00 0.60 1.60 2.00 0.70 -0.50 2.00 0.50 -0.50 2.00 1.70 -0.10 1.70 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 1.40 1.20 -0.60 2.00 1.10 1.80 2.00 0.80 0.00 2.00 1.10 -0.70 2.00 1.80 -0.30 1.40 1.70 0.40 0.50 2.00 0.40 1.40 2.00 0.50 1.10 2.00 1.30 -2.50 2.00 1.50 -2.10 1.50 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.40 1.60 0.80 2.00 1.50 1.60 2.00 0.60 1.40 2.00 2.10 -0.30 2.00 1.60 0.10 0.80 0.70 -0.50 -0.50 2.00 -0.60 0.50 2.00 -0.50 0.10 2.00 0.40 -3.50 2.00 0.50 -3.10 0.50 2.00 0.80 0.80 2.00 0.70 0.80 2.00 -0.20 1.50 2.00 1.70 -0.30 2.00 0.80 0.10 0.00 Y --------------------------------------------------------------------------------------------- A A A A C C C C G G G G U U U U A C G U A C G U A C G U A C G U --------------------------------------------------------------------------------------------- 5' ------> 3' A \/ \_/ G U /\ | C 3' <------ 5' 2.00 1.90 1.00 2.00 2.40 2.80 2.00 2.70 1.00 2.00 1.80 0.30 2.00 2.70 1.80 2.20 1.90 1.80 0.90 2.00 2.20 2.10 2.00 1.90 0.90 2.00 1.60 -0.80 2.00 1.90 0.70 1.00 0.80 0.70 -0.20 2.00 1.10 2.00 2.00 1.80 -0.20 2.00 0.50 -0.10 2.00 1.80 1.40 1.80 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 1.80 1.70 0.80 2.00 2.10 2.00 2.00 1.80 0.80 2.00 1.50 -0.90 2.00 1.80 0.60 0.90 2.30 1.60 1.70 2.00 2.10 1.90 2.00 1.80 1.70 2.00 2.10 0.00 2.00 1.80 1.50 0.90 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.30 1.60 1.70 2.00 2.10 1.90 2.00 1.80 1.70 2.00 2.10 0.00 2.00 1.80 1.50 0.90 0.80 0.70 -0.20 2.00 1.10 2.00 2.00 1.80 -0.20 2.00 0.50 -0.10 2.00 1.80 1.40 1.80 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 1.30 1.20 0.30 2.00 1.70 2.10 2.00 2.00 0.30 2.00 1.10 -0.40 2.00 2.00 1.10 1.50 1.60 0.50 1.40 2.00 0.90 1.80 2.00 1.60 1.40 2.00 1.30 -2.10 2.00 1.60 -0.60 1.60 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.30 1.60 1.70 2.00 2.10 1.90 2.00 1.80 1.70 2.00 2.10 0.00 2.00 1.80 1.50 0.90 0.60 -0.50 0.40 2.00 0.00 0.80 2.00 0.70 0.40 2.00 0.40 -3.10 2.00 0.70 -1.60 0.60 1.90 0.80 1.80 2.00 1.30 1.10 2.00 1.00 1.80 2.00 1.70 0.00 2.00 1.00 1.60 0.10 Y --------------------------------------------------------------------------------------------- A A A A C C C C G G G G U U U U A C G U A C G U A C G U A C G U --------------------------------------------------------------------------------------------- 5' ------> 3' A \/ \_/ U U /\ | A 3' <------ 5' 2.80 2.50 1.50 2.00 2.60 3.10 2.00 3.10 1.50 2.00 2.10 1.30 2.00 3.10 2.30 2.70 2.60 2.40 1.40 2.00 2.50 2.30 2.00 2.30 1.40 2.00 1.90 0.20 2.00 2.30 1.20 1.50 1.50 1.30 0.30 2.00 1.40 2.20 2.00 2.20 0.30 2.00 0.80 0.90 2.00 2.20 1.90 2.20 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.50 2.30 1.30 2.00 2.40 2.20 2.00 2.20 1.30 2.00 1.80 0.10 2.00 2.20 1.10 1.40 3.10 2.20 2.20 2.00 2.30 2.20 2.00 2.20 2.20 2.00 2.40 1.00 2.00 2.20 2.00 1.40 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 3.10 2.20 2.20 2.00 2.30 2.20 2.00 2.20 2.20 2.00 2.40 1.00 2.00 2.20 2.00 1.40 1.50 1.30 0.30 2.00 1.40 2.20 2.00 2.20 0.30 2.00 0.80 0.90 2.00 2.20 1.90 2.20 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.10 1.80 0.80 2.00 1.90 2.40 2.00 2.40 0.80 2.00 1.40 0.70 2.00 2.40 1.60 2.00 2.30 1.10 1.90 2.00 1.20 2.00 2.00 2.00 1.90 2.00 1.60 -1.10 2.00 2.00 -0.10 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 3.10 2.20 2.20 2.00 2.30 2.20 2.00 2.20 2.20 2.00 2.40 1.00 2.00 2.20 2.00 1.40 1.30 0.10 0.90 2.00 0.20 1.00 2.00 1.00 0.90 2.00 0.70 -2.10 2.00 1.00 -1.10 1.10 2.70 1.40 2.20 2.00 1.50 1.40 2.00 1.40 2.20 2.00 2.00 1.10 2.00 1.40 2.00 0.60 Y --------------------------------------------------------------------------------------------- A A A A C C C C G G G G U U U U A C G U A C G U A C G U A C G U --------------------------------------------------------------------------------------------- 5' ------> 3' A \/ \_/ G U /\ | U 3' <------ 5' 2.80 2.30 1.70 2.00 2.80 3.40 2.00 3.40 1.70 2.00 2.10 1.00 2.00 3.10 2.20 2.90 2.60 2.20 1.60 2.00 2.60 2.60 2.00 2.60 1.60 2.00 2.00 -0.20 2.00 2.30 1.10 1.80 1.50 1.10 0.50 2.00 1.50 2.50 2.00 2.50 0.50 2.00 0.90 0.50 2.00 2.20 1.80 2.50 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.50 2.10 1.50 2.00 2.50 2.50 2.00 2.50 1.50 2.00 1.90 -0.30 2.00 2.20 1.00 1.70 3.10 2.00 2.40 2.00 2.50 2.50 2.00 2.50 2.40 2.00 2.40 0.70 2.00 2.20 1.90 1.60 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 3.10 2.00 2.40 2.00 2.50 2.50 2.00 2.50 2.40 2.00 2.40 0.70 2.00 2.20 1.90 1.60 1.50 1.10 0.50 2.00 1.50 2.50 2.00 2.50 0.50 2.00 0.90 0.50 2.00 2.20 1.80 2.50 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.10 1.60 1.00 2.00 2.10 2.70 2.00 2.70 1.00 2.00 1.40 0.30 2.00 2.40 1.50 2.20 2.30 0.90 2.10 2.00 1.30 2.30 2.00 2.30 2.10 2.00 1.70 -1.50 2.00 2.00 -0.20 2.30 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 3.10 2.00 2.40 2.00 2.50 2.50 2.00 2.50 2.40 2.00 2.40 0.70 2.00 2.20 1.90 1.60 1.30 -0.10 1.10 2.00 0.30 1.30 2.00 1.30 1.10 2.00 0.70 -2.50 2.00 1.00 -1.20 1.30 2.70 1.20 2.40 2.00 1.70 1.70 2.00 1.70 2.40 2.00 2.00 0.70 2.00 1.40 1.90 0.80 Y --------------------------------------------------------------------------------------------- A A A A C C C C G G G G U U U U A C G U A C G U A C G U A C G U --------------------------------------------------------------------------------------------- 5' ------> 3' A \/ \_/ U U /\ | G 3' <------ 5' 2.80 2.50 1.50 2.00 2.60 3.10 2.00 3.10 1.50 2.00 2.10 1.30 2.00 3.10 2.30 2.70 2.60 2.40 1.40 2.00 2.50 2.30 2.00 2.30 1.40 2.00 1.90 0.20 2.00 2.30 1.20 1.50 1.50 1.30 0.30 2.00 1.40 2.20 2.00 2.20 0.30 2.00 0.80 0.90 2.00 2.20 1.90 2.20 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.50 2.30 1.30 2.00 2.40 2.20 2.00 2.20 1.30 2.00 1.80 0.10 2.00 2.20 1.10 1.40 3.10 2.20 2.20 2.00 2.30 2.20 2.00 2.20 2.20 2.00 2.40 1.00 2.00 2.20 2.00 1.40 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 3.10 2.20 2.20 2.00 2.30 2.20 2.00 2.20 2.20 2.00 2.40 1.00 2.00 2.20 2.00 1.40 1.50 1.30 0.30 2.00 1.40 2.20 2.00 2.20 0.30 2.00 0.80 0.90 2.00 2.20 1.90 2.20 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.10 1.80 0.80 2.00 1.90 2.40 2.00 2.40 0.80 2.00 1.40 0.70 2.00 2.40 1.60 2.00 2.30 1.10 1.90 2.00 1.20 2.00 2.00 2.00 1.90 2.00 1.60 -1.10 2.00 2.00 -0.10 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 3.10 2.20 2.20 2.00 2.30 2.20 2.00 2.20 2.20 2.00 2.40 1.00 2.00 2.20 2.00 1.40 1.30 0.10 0.90 2.00 0.20 1.00 2.00 1.00 0.90 2.00 0.70 -2.10 2.00 1.00 -1.10 1.10 2.70 1.40 2.20 2.00 1.50 1.40 2.00 1.40 2.20 2.00 2.00 1.10 2.00 1.40 2.00 0.60 Y --------------------------------------------------------------------------------------------- A A A A C C C C G G G G U U U U A C G U A C G U A C G U A C G U --------------------------------------------------------------------------------------------- 5' ------> 3' C \/ \_/ A G /\ | U 3' <------ 5' 2.00 1.60 1.00 2.00 2.00 2.60 2.00 2.60 1.00 2.00 1.40 0.20 2.00 2.30 1.50 2.20 2.40 1.90 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2.00 2.00 0.30 2.00 1.80 1.50 1.20 0.30 -1.10 0.10 2.00 -0.70 0.30 2.00 0.30 0.10 2.00 -0.30 -3.50 2.00 0.00 -2.20 0.30 2.20 0.70 1.90 2.00 1.20 1.20 2.00 1.20 1.90 2.00 1.50 0.20 2.00 0.90 1.50 0.30 Y --------------------------------------------------------------------------------------------- A A A A C C C C G G G G U U U U A C G U A C G U A C G U A C G U --------------------------------------------------------------------------------------------- 5' ------> 3' C \/ \_/ C G /\ | G 3' <------ 5' 0.50 1.10 -0.30 2.00 1.10 1.70 2.00 0.70 0.40 2.00 1.00 0.10 2.00 1.80 -0.50 1.50 0.60 1.50 0.10 2.00 1.10 1.50 2.00 0.50 0.50 2.00 1.40 -0.70 2.00 1.50 -0.60 0.00 0.00 -0.70 -1.60 2.00 -1.00 -0.60 2.00 0.20 -0.70 2.00 0.00 -0.80 2.00 1.20 -0.60 0.90 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 1.30 1.00 -0.70 2.00 1.00 1.00 2.00 0.00 0.70 2.00 0.90 -1.90 2.00 1.10 -1.50 -0.20 2.20 1.30 0.70 2.00 1.90 1.30 2.00 0.30 0.70 2.00 1.80 -0.30 2.00 1.40 -0.20 -0.10 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--------------------------------------------------------------------------------------------- 5' ------> 3' C \/ \_/ G G /\ | C 3' <------ 5' 1.30 1.20 0.30 2.00 1.60 2.10 2.00 1.90 0.30 2.00 1.00 -0.40 2.00 1.90 1.10 1.40 1.60 1.50 0.60 2.00 2.00 1.80 2.00 1.70 0.60 2.00 1.40 -1.10 2.00 1.70 0.40 0.80 0.30 0.20 -0.70 2.00 0.60 1.50 2.00 1.30 -0.70 2.00 0.00 -0.60 2.00 1.30 0.90 1.30 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 1.20 1.10 0.20 2.00 1.50 1.40 2.00 1.20 0.20 2.00 0.90 -1.50 2.00 1.20 0.00 0.30 2.10 1.40 1.50 2.00 1.80 1.70 2.00 1.50 1.50 2.00 1.80 -0.20 2.00 1.50 1.30 0.60 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 1.90 1.20 1.30 2.00 1.70 1.50 2.00 1.40 1.30 2.00 1.70 -0.40 2.00 1.40 1.10 0.50 0.30 0.20 -0.70 2.00 0.60 1.50 2.00 1.30 -0.70 2.00 0.00 -0.60 2.00 1.30 0.90 1.30 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 1.00 0.90 0.00 2.00 1.40 1.80 2.00 1.70 0.00 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0.90 2.00 0.90 1.80 2.00 1.50 0.60 2.00 0.90 1.60 0.10 Y --------------------------------------------------------------------------------------------- A A A A C C C C G G G G U U U U A C G U A C G U A C G U A C G U --------------------------------------------------------------------------------------------- 5' ------> 3' C \/ \_/ G G /\ | U 3' <------ 5' 2.00 1.60 1.00 2.00 2.00 2.60 2.00 2.60 1.00 2.00 1.40 0.20 2.00 2.30 1.50 2.20 2.40 1.90 1.30 2.00 2.40 2.40 2.00 2.40 1.30 2.00 1.70 -0.40 2.00 2.10 0.80 1.50 1.00 0.60 0.00 2.00 1.00 2.00 2.00 2.00 0.00 2.00 0.40 0.00 2.00 1.70 1.30 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 1.90 1.50 0.90 2.00 1.90 1.90 2.00 1.90 0.90 2.00 1.30 -0.90 2.00 1.60 0.40 1.10 2.80 1.80 2.20 2.00 2.20 2.20 2.00 2.20 2.20 2.00 2.20 0.40 2.00 1.90 1.70 1.40 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.70 1.60 2.00 2.00 2.10 2.10 2.00 2.10 2.00 2.00 2.00 0.30 2.00 1.80 1.50 1.20 1.00 0.60 0.00 2.00 1.00 2.00 2.00 2.00 0.00 2.00 0.40 0.00 2.00 1.70 1.30 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 1.80 1.30 0.70 2.00 1.80 2.40 2.00 2.40 0.70 2.00 1.10 0.00 2.00 2.10 1.20 1.90 1.80 0.40 1.60 2.00 0.80 1.80 2.00 1.80 1.60 2.00 1.20 -2.00 2.00 1.50 -0.70 1.80 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.70 1.60 2.00 2.00 2.10 2.10 2.00 2.10 2.00 2.00 2.00 0.30 2.00 1.80 1.50 1.20 0.30 -1.10 0.10 2.00 -0.70 0.30 2.00 0.30 0.10 2.00 -0.30 -3.50 2.00 0.00 -2.20 0.30 2.20 0.70 1.90 2.00 1.20 1.20 2.00 1.20 1.90 2.00 1.50 0.20 2.00 0.90 1.50 0.30 Y --------------------------------------------------------------------------------------------- A A A A C C C C G G G G U U U U A C G U A C G U A C G U A C G U --------------------------------------------------------------------------------------------- 5' ------> 3' C \/ \_/ U G /\ | G 3' <------ 5' 2.00 1.80 0.80 2.00 1.90 2.30 2.00 2.30 0.80 2.00 1.30 0.60 2.00 2.30 1.60 1.90 2.40 2.10 1.10 2.00 2.20 2.10 2.00 2.10 1.10 2.00 1.70 0.00 2.00 2.10 0.90 1.30 1.00 0.80 -0.20 2.00 0.90 1.70 2.00 1.70 -0.20 2.00 0.30 0.40 2.00 1.70 1.40 1.80 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 1.90 1.70 0.70 2.00 1.80 1.60 2.00 1.60 0.70 2.00 1.20 -0.50 2.00 1.60 0.50 0.80 2.80 2.00 2.00 2.00 2.10 1.90 2.00 1.90 2.00 2.00 2.10 0.80 2.00 1.90 1.80 1.10 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.70 1.80 1.80 2.00 1.90 1.80 2.00 1.80 1.80 2.00 2.00 0.70 2.00 1.80 1.60 1.00 1.00 0.80 -0.20 2.00 0.90 1.70 2.00 1.70 -0.20 2.00 0.30 0.40 2.00 1.70 1.40 1.80 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 1.80 1.50 0.50 2.00 1.60 2.10 2.00 2.10 0.50 2.00 1.10 0.40 2.00 2.10 1.30 1.70 1.80 0.60 1.40 2.00 0.70 1.50 2.00 1.50 1.40 2.00 1.10 -1.60 2.00 1.50 -0.60 1.60 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 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2.00 1.80 0.10 2.00 1.60 1.30 1.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.50 1.50 1.90 2.00 1.90 1.90 2.00 1.90 1.90 2.00 1.90 0.10 2.00 1.60 1.40 1.10 0.10 -0.30 -0.90 2.00 0.10 1.10 2.00 1.10 -0.90 2.00 -0.50 -0.90 2.00 0.80 0.40 1.10 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 1.80 1.30 0.70 2.00 1.80 2.40 2.00 2.40 0.70 2.00 1.10 0.00 2.00 2.10 1.20 1.90 0.40 -1.10 0.10 2.00 -0.60 0.40 2.00 0.40 0.10 2.00 -0.30 -3.50 2.00 0.10 -2.20 0.30 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 1.50 0.40 0.90 2.00 0.90 0.90 2.00 0.90 0.90 2.00 0.80 -0.90 2.00 0.60 0.40 0.00 0.00 -1.50 -0.30 2.00 -1.00 0.00 2.00 0.00 -0.30 2.00 -0.70 -3.90 2.00 -0.30 -2.60 -0.10 2.10 0.70 1.90 2.00 1.10 1.10 2.00 1.10 1.90 2.00 1.50 0.10 2.00 0.80 1.40 0.30 Y --------------------------------------------------------------------------------------------- A A A A C C C C G G G G U U U U A C G U A C G U A C G U A C G U --------------------------------------------------------------------------------------------- 5' ------> 3' G \/ \_/ C C /\ | G 3' <------ 5' 1.50 1.20 -0.50 2.00 1.20 1.80 2.00 0.80 0.10 2.00 1.10 -0.70 2.00 1.90 -0.30 1.50 1.20 0.90 -0.80 2.00 0.90 0.90 2.00 0.00 -0.20 2.00 0.80 -2.00 2.00 1.00 -1.60 0.20 0.10 -0.10 -1.90 2.00 -0.20 0.90 2.00 -0.10 -1.30 2.00 -0.20 -1.30 2.00 0.90 -0.90 0.90 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 1.20 1.00 -0.80 2.00 0.90 1.00 2.00 0.00 -0.10 2.00 0.90 -1.90 2.00 1.00 -1.50 0.20 1.80 1.00 0.20 2.00 0.90 1.00 2.00 0.00 0.90 2.00 1.50 -0.90 2.00 1.00 -0.50 0.20 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 1.90 1.00 0.30 2.00 1.00 1.00 2.00 0.00 0.90 2.00 1.50 -0.90 2.00 1.10 -0.50 0.30 -0.50 -0.80 -2.60 2.00 -0.80 0.20 2.00 -0.80 -1.90 2.00 -0.90 -1.90 2.00 0.30 -1.50 0.30 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 1.10 0.90 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--------------------------------------------------------------------------------------------- 5' ------> 3' G \/ \_/ G C /\ | U 3' <------ 5' 2.10 1.70 1.10 2.00 2.10 2.70 2.00 2.70 1.10 2.00 1.50 0.30 2.00 2.40 1.60 2.30 1.80 1.40 0.80 2.00 1.80 1.80 2.00 1.80 0.80 2.00 1.20 -1.00 2.00 1.50 0.30 1.00 0.70 0.30 -0.30 2.00 0.70 1.70 2.00 1.70 -0.30 2.00 0.10 -0.30 2.00 1.40 1.00 1.70 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 1.90 1.40 0.80 2.00 1.90 1.90 2.00 1.90 0.80 2.00 1.20 -0.90 2.00 1.60 0.30 1.00 2.50 1.40 1.80 2.00 1.90 1.90 2.00 1.90 1.80 2.00 1.80 0.10 2.00 1.60 1.30 1.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.50 1.50 1.90 2.00 1.90 1.90 2.00 1.90 1.90 2.00 1.90 0.10 2.00 1.60 1.40 1.10 0.10 -0.30 -0.90 2.00 0.10 1.10 2.00 1.10 -0.90 2.00 -0.50 -0.90 2.00 0.80 0.40 1.10 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 1.80 1.30 0.70 2.00 1.80 2.40 2.00 2.40 0.70 2.00 1.10 0.00 2.00 2.10 1.20 1.90 0.40 -1.10 0.10 2.00 -0.60 0.40 2.00 0.40 0.10 2.00 -0.30 -3.50 2.00 0.10 -2.20 0.30 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 1.50 0.40 0.90 2.00 0.90 0.90 2.00 0.90 0.90 2.00 0.80 -0.90 2.00 0.60 0.40 0.00 0.00 -1.50 -0.30 2.00 -1.00 0.00 2.00 0.00 -0.30 2.00 -0.70 -3.90 2.00 -0.30 -2.60 -0.10 2.10 0.70 1.90 2.00 1.10 1.10 2.00 1.10 1.90 2.00 1.50 0.10 2.00 0.80 1.40 0.30 Y --------------------------------------------------------------------------------------------- A A A A C C C C G G G G U U U U A C G U A C G U A C G U A C G U --------------------------------------------------------------------------------------------- 5' ------> 3' G \/ \_/ U C /\ | G 3' <------ 5' 2.10 1.90 0.90 2.00 2.00 2.40 2.00 2.40 0.90 2.00 1.40 0.70 2.00 2.40 1.70 2.00 1.80 1.60 0.60 2.00 1.70 1.50 2.00 1.50 0.60 2.00 1.10 -0.60 2.00 1.50 0.40 0.70 0.70 0.50 -0.50 2.00 0.60 1.40 2.00 1.40 -0.50 2.00 0.00 0.10 2.00 1.40 1.10 1.50 2.00 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1.00 0.80 2.00 0.80 1.70 2.00 1.40 0.50 2.00 0.80 1.50 0.00 Y --------------------------------------------------------------------------------------------- A A A A C C C C G G G G U U U U A C G U A C G U A C G U A C G U --------------------------------------------------------------------------------------------- 5' ------> 3' U \/ \_/ A A /\ | U 3' <------ 5' 2.80 2.30 1.70 2.00 2.80 3.40 2.00 3.40 1.70 2.00 2.10 1.00 2.00 3.10 2.20 2.90 2.80 2.30 1.70 2.00 2.80 2.80 2.00 2.80 1.70 2.00 2.10 0.00 2.00 2.50 1.20 1.90 1.70 1.30 0.70 2.00 1.70 2.70 2.00 2.70 0.70 2.00 1.10 0.70 2.00 2.40 2.00 2.70 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.30 1.90 1.30 2.00 2.30 2.30 2.00 2.30 1.30 2.00 1.70 -0.50 2.00 2.00 0.80 1.50 3.40 2.30 2.70 2.00 2.80 2.80 2.00 2.80 2.70 2.00 2.70 1.00 2.00 2.50 2.20 1.90 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 3.10 2.00 2.40 2.00 2.50 2.50 2.00 2.50 2.40 2.00 2.40 0.70 2.00 2.20 1.90 1.60 1.70 1.30 0.70 2.00 1.70 2.70 2.00 2.70 0.70 2.00 1.10 0.70 2.00 2.40 2.00 2.70 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.10 1.70 1.10 2.00 2.10 2.70 2.00 2.70 1.10 2.00 1.50 0.30 2.00 2.40 1.60 2.30 2.20 0.80 2.00 2.00 1.20 2.20 2.00 2.20 2.00 2.00 1.60 -1.60 2.00 1.90 -0.30 2.20 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 3.40 2.30 2.70 2.00 2.80 2.80 2.00 2.80 2.70 2.00 2.70 1.00 2.00 2.50 2.20 1.90 1.00 -0.50 0.70 2.00 0.00 1.00 2.00 1.00 0.70 2.00 0.30 -2.90 2.00 0.70 -1.60 0.90 2.90 1.50 2.70 2.00 1.90 1.90 2.00 1.90 2.70 2.00 2.30 0.90 2.00 1.60 2.20 1.10 Y --------------------------------------------------------------------------------------------- A A A A C C C C G G G G U U U U A C G U A C G U A C G U A C G U --------------------------------------------------------------------------------------------- 5' ------> 3' U \/ \_/ C A /\ | G 3' <------ 5' 2.10 1.90 0.10 2.00 1.80 2.50 2.00 1.50 0.70 2.00 1.80 0.00 2.00 2.50 0.40 2.10 2.10 1.90 0.10 2.00 1.80 1.90 2.00 0.90 0.70 2.00 1.80 -1.00 2.00 1.90 -0.60 1.10 1.10 0.80 -0.90 2.00 0.80 1.80 2.00 0.90 -0.30 2.00 0.70 -0.30 2.00 1.90 0.10 1.90 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 1.70 1.40 -0.30 2.00 1.40 1.40 2.00 0.40 0.30 2.00 1.30 -1.50 2.00 1.50 -1.10 0.70 2.70 1.90 1.10 2.00 1.80 1.90 2.00 0.90 1.70 2.00 2.40 0.00 2.00 1.90 0.40 1.10 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.40 1.60 0.80 2.00 1.50 1.60 2.00 0.60 1.40 2.00 2.10 -0.30 2.00 1.60 0.10 0.80 1.10 0.80 -0.90 2.00 0.80 1.80 2.00 0.90 -0.30 2.00 0.70 -0.30 2.00 1.90 0.10 1.90 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 1.50 1.20 -0.50 2.00 1.20 1.80 2.00 0.80 0.10 2.00 1.10 -0.70 2.00 1.90 -0.30 1.50 1.60 0.30 0.40 2.00 0.30 1.30 2.00 0.40 1.00 2.00 1.20 -2.60 2.00 1.40 -2.20 1.40 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.70 1.90 1.10 2.00 1.80 1.90 2.00 0.90 1.70 2.00 2.40 0.00 2.00 1.90 0.40 1.10 0.30 -0.90 -0.90 2.00 -1.00 0.10 2.00 -0.90 -0.30 2.00 0.00 -3.90 2.00 0.10 -3.50 0.10 2.30 1.00 1.10 2.00 1.00 1.00 2.00 0.00 1.70 2.00 1.90 -0.10 2.00 1.10 0.30 0.30 Y --------------------------------------------------------------------------------------------- A A A A C C C C G G G G U U U U A C G U A C G U A C G U A C G U --------------------------------------------------------------------------------------------- 5' ------> 3' U \/ \_/ G A /\ | C 3' <------ 5' 2.00 1.90 1.00 2.00 2.40 2.80 2.00 2.70 1.00 2.00 1.80 0.30 2.00 2.70 1.80 2.20 2.00 1.90 1.00 2.00 2.40 2.20 2.00 2.10 1.00 2.00 1.80 -0.70 2.00 2.10 0.80 1.20 1.00 0.90 0.00 2.00 1.30 2.20 2.00 2.00 0.00 2.00 0.70 0.10 2.00 2.00 1.60 1.90 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 1.60 1.50 0.60 2.00 1.90 1.80 2.00 1.60 0.60 2.00 1.30 -1.10 2.00 1.60 0.40 0.70 2.60 1.90 2.00 2.00 2.40 2.20 2.00 2.10 2.00 2.00 2.40 0.30 2.00 2.10 1.80 1.20 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.30 1.60 1.70 2.00 2.10 1.90 2.00 1.80 1.70 2.00 2.10 0.00 2.00 1.80 1.50 0.90 1.00 0.90 0.00 2.00 1.30 2.20 2.00 2.00 0.00 2.00 0.70 0.10 2.00 2.00 1.60 1.90 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 1.40 1.30 0.40 2.00 1.70 2.20 2.00 2.00 0.40 2.00 1.10 -0.30 2.00 2.00 1.20 1.50 1.50 0.40 1.30 2.00 0.80 1.70 2.00 1.50 1.30 2.00 1.20 -2.20 2.00 1.50 -0.70 1.50 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.60 1.90 2.00 2.00 2.40 2.20 2.00 2.10 2.00 2.00 2.40 0.30 2.00 2.10 1.80 1.20 0.20 -0.90 0.00 2.00 -0.40 0.40 2.00 0.30 0.00 2.00 0.00 -3.50 2.00 0.30 -2.00 0.20 2.20 1.10 2.00 2.00 1.50 1.40 2.00 1.20 2.00 2.00 1.90 0.30 2.00 1.20 1.80 0.30 Y --------------------------------------------------------------------------------------------- A A A A C C C C G G G G U U U U A C G U A C G U A C G U A C G U --------------------------------------------------------------------------------------------- 5' ------> 3' U \/ \_/ U A /\ | A 3' <------ 5' 2.80 2.50 1.50 2.00 2.60 3.10 2.00 3.10 1.50 2.00 2.10 1.30 2.00 3.10 2.30 2.70 2.80 2.50 1.50 2.00 2.60 2.50 2.00 2.50 1.50 2.00 2.10 0.30 2.00 2.50 1.30 1.70 1.70 1.50 0.50 2.00 1.60 2.40 2.00 2.40 0.50 2.00 1.00 1.10 2.00 2.40 2.10 2.40 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.30 2.10 1.10 2.00 2.20 2.00 2.00 2.00 1.10 2.00 1.60 -0.10 2.00 2.00 0.90 1.20 3.40 2.50 2.50 2.00 2.60 2.50 2.00 2.50 2.50 2.00 2.70 1.30 2.00 2.50 2.30 1.70 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 3.10 2.20 2.20 2.00 2.30 2.20 2.00 2.20 2.20 2.00 2.40 1.00 2.00 2.20 2.00 1.40 1.70 1.50 0.50 2.00 1.60 2.40 2.00 2.40 0.50 2.00 1.00 1.10 2.00 2.40 2.10 2.40 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.10 1.90 0.90 2.00 2.00 2.40 2.00 2.40 0.90 2.00 1.40 0.70 2.00 2.40 1.70 2.00 2.20 1.00 1.80 2.00 1.10 1.90 2.00 1.90 1.80 2.00 1.50 -1.20 2.00 1.90 -0.20 1.90 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 3.40 2.50 2.50 2.00 2.60 2.50 2.00 2.50 2.50 2.00 2.70 1.30 2.00 2.50 2.30 1.70 1.00 -0.30 0.50 2.00 -0.20 0.70 2.00 0.70 0.50 2.00 0.30 -2.50 2.00 0.70 -1.50 0.70 2.90 1.70 2.50 2.00 1.80 1.60 2.00 1.60 2.50 2.00 2.20 1.30 2.00 1.60 2.30 0.80 Y --------------------------------------------------------------------------------------------- A A A A C C C C G G G G U U U U A C G U A C G U A C G U A C G U --------------------------------------------------------------------------------------------- 5' ------> 3' U \/ \_/ G A /\ | U 3' <------ 5' 2.80 2.30 1.70 2.00 2.80 3.40 2.00 3.40 1.70 2.00 2.10 1.00 2.00 3.10 2.20 2.90 2.80 2.30 1.70 2.00 2.80 2.80 2.00 2.80 1.70 2.00 2.10 0.00 2.00 2.50 1.20 1.90 1.70 1.30 0.70 2.00 1.70 2.70 2.00 2.70 0.70 2.00 1.10 0.70 2.00 2.40 2.00 2.70 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.30 1.90 1.30 2.00 2.30 2.30 2.00 2.30 1.30 2.00 1.70 -0.50 2.00 2.00 0.80 1.50 3.40 2.30 2.70 2.00 2.80 2.80 2.00 2.80 2.70 2.00 2.70 1.00 2.00 2.50 2.20 1.90 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 3.10 2.00 2.40 2.00 2.50 2.50 2.00 2.50 2.40 2.00 2.40 0.70 2.00 2.20 1.90 1.60 1.70 1.30 0.70 2.00 1.70 2.70 2.00 2.70 0.70 2.00 1.10 0.70 2.00 2.40 2.00 2.70 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.10 1.70 1.10 2.00 2.10 2.70 2.00 2.70 1.10 2.00 1.50 0.30 2.00 2.40 1.60 2.30 2.20 0.80 2.00 2.00 1.20 2.20 2.00 2.20 2.00 2.00 1.60 -1.60 2.00 1.90 -0.30 2.20 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 3.40 2.30 2.70 2.00 2.80 2.80 2.00 2.80 2.70 2.00 2.70 1.00 2.00 2.50 2.20 1.90 1.00 -0.50 0.70 2.00 0.00 1.00 2.00 1.00 0.70 2.00 0.30 -2.90 2.00 0.70 -1.60 0.90 2.90 1.50 2.70 2.00 1.90 1.90 2.00 1.90 2.70 2.00 2.30 0.90 2.00 1.60 2.20 1.10 Y --------------------------------------------------------------------------------------------- A A A A C C C C G G G G U U U U A C G U A C G U A C G U A C G U --------------------------------------------------------------------------------------------- 5' ------> 3' U \/ \_/ U A /\ | G 3' <------ 5' 2.80 2.50 1.50 2.00 2.60 3.10 2.00 3.10 1.50 2.00 2.10 1.30 2.00 3.10 2.30 2.70 2.80 2.50 1.50 2.00 2.60 2.50 2.00 2.50 1.50 2.00 2.10 0.30 2.00 2.50 1.30 1.70 1.70 1.50 0.50 2.00 1.60 2.40 2.00 2.40 0.50 2.00 1.00 1.10 2.00 2.40 2.10 2.40 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.30 2.10 1.10 2.00 2.20 2.00 2.00 2.00 1.10 2.00 1.60 -0.10 2.00 2.00 0.90 1.20 3.40 2.50 2.50 2.00 2.60 2.50 2.00 2.50 2.50 2.00 2.70 1.30 2.00 2.50 2.30 1.70 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 3.10 2.20 2.20 2.00 2.30 2.20 2.00 2.20 2.20 2.00 2.40 1.00 2.00 2.20 2.00 1.40 1.70 1.50 0.50 2.00 1.60 2.40 2.00 2.40 0.50 2.00 1.00 1.10 2.00 2.40 2.10 2.40 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.10 1.90 0.90 2.00 2.00 2.40 2.00 2.40 0.90 2.00 1.40 0.70 2.00 2.40 1.70 2.00 2.20 1.00 1.80 2.00 1.10 1.90 2.00 1.90 1.80 2.00 1.50 -1.20 2.00 1.90 -0.20 1.90 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 3.40 2.50 2.50 2.00 2.60 2.50 2.00 2.50 2.50 2.00 2.70 1.30 2.00 2.50 2.30 1.70 1.00 -0.30 0.50 2.00 -0.20 0.70 2.00 0.70 0.50 2.00 0.30 -2.50 2.00 0.70 -1.50 0.70 2.90 1.70 2.50 2.00 1.80 1.60 2.00 1.60 2.50 2.00 2.20 1.30 2.00 1.60 2.30 0.80 Y --------------------------------------------------------------------------------------------- A A A A C C C C G G G G U U U U A C G U A C G U A C G U A C G U --------------------------------------------------------------------------------------------- 5' ------> 3' G \/ \_/ A U /\ | U 3' <------ 5' 2.80 2.30 1.70 2.00 2.80 3.40 2.00 3.40 1.70 2.00 2.10 1.00 2.00 3.10 2.20 2.90 2.60 2.20 1.60 2.00 2.60 2.60 2.00 2.60 1.60 2.00 2.00 -0.20 2.00 2.30 1.10 1.80 1.50 1.10 0.50 2.00 1.50 2.50 2.00 2.50 0.50 2.00 0.90 0.50 2.00 2.20 1.80 2.50 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.50 2.10 1.50 2.00 2.50 2.50 2.00 2.50 1.50 2.00 1.90 -0.30 2.00 2.20 1.00 1.70 3.10 2.00 2.40 2.00 2.50 2.50 2.00 2.50 2.40 2.00 2.40 0.70 2.00 2.20 1.90 1.60 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 3.10 2.00 2.40 2.00 2.50 2.50 2.00 2.50 2.40 2.00 2.40 0.70 2.00 2.20 1.90 1.60 1.50 1.10 0.50 2.00 1.50 2.50 2.00 2.50 0.50 2.00 0.90 0.50 2.00 2.20 1.80 2.50 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.10 1.60 1.00 2.00 2.10 2.70 2.00 2.70 1.00 2.00 1.40 0.30 2.00 2.40 1.50 2.20 2.30 0.90 2.10 2.00 1.30 2.30 2.00 2.30 2.10 2.00 1.70 -1.50 2.00 2.00 -0.20 2.30 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 3.10 2.00 2.40 2.00 2.50 2.50 2.00 2.50 2.40 2.00 2.40 0.70 2.00 2.20 1.90 1.60 1.30 -0.10 1.10 2.00 0.30 1.30 2.00 1.30 1.10 2.00 0.70 -2.50 2.00 1.00 -1.20 1.30 2.70 1.20 2.40 2.00 1.70 1.70 2.00 1.70 2.40 2.00 2.00 0.70 2.00 1.40 1.90 0.80 Y --------------------------------------------------------------------------------------------- A A A A C C C C G G G G U U U U A C G U A C G U A C G U A C G U --------------------------------------------------------------------------------------------- 5' ------> 3' G \/ \_/ C U /\ | G 3' <------ 5' 2.10 1.90 0.10 2.00 1.80 2.50 2.00 1.50 0.70 2.00 1.80 0.00 2.00 2.50 0.40 2.10 2.00 1.70 0.00 2.00 1.70 1.70 2.00 0.70 0.60 2.00 1.60 -1.20 2.00 1.80 -0.80 1.00 0.90 0.60 -1.10 2.00 0.60 1.60 2.00 0.70 -0.50 2.00 0.50 -0.50 2.00 1.70 -0.10 1.70 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 1.90 1.60 -0.10 2.00 1.60 1.60 2.00 0.60 0.50 2.00 1.50 -1.30 2.00 1.70 -0.90 0.90 2.40 1.60 0.80 2.00 1.50 1.60 2.00 0.60 1.40 2.00 2.10 -0.30 2.00 1.60 0.10 0.80 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.40 1.60 0.80 2.00 1.50 1.60 2.00 0.60 1.40 2.00 2.10 -0.30 2.00 1.60 0.10 0.80 0.90 0.60 -1.10 2.00 0.60 1.60 2.00 0.70 -0.50 2.00 0.50 -0.50 2.00 1.70 -0.10 1.70 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 1.40 1.20 -0.60 2.00 1.10 1.80 2.00 0.80 0.00 2.00 1.10 -0.70 2.00 1.80 -0.30 1.40 1.70 0.40 0.50 2.00 0.40 1.40 2.00 0.50 1.10 2.00 1.30 -2.50 2.00 1.50 -2.10 1.50 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.40 1.60 0.80 2.00 1.50 1.60 2.00 0.60 1.40 2.00 2.10 -0.30 2.00 1.60 0.10 0.80 0.70 -0.50 -0.50 2.00 -0.60 0.50 2.00 -0.50 0.10 2.00 0.40 -3.50 2.00 0.50 -3.10 0.50 2.00 0.80 0.80 2.00 0.70 0.80 2.00 -0.20 1.50 2.00 1.70 -0.30 2.00 0.80 0.10 0.00 Y --------------------------------------------------------------------------------------------- A A A A C C C C G G G G U U U U A C G U A C G U A C G U A C G U --------------------------------------------------------------------------------------------- 5' ------> 3' G \/ \_/ G U /\ | C 3' <------ 5' 2.00 1.90 1.00 2.00 2.40 2.80 2.00 2.70 1.00 2.00 1.80 0.30 2.00 2.70 1.80 2.20 1.90 1.80 0.90 2.00 2.20 2.10 2.00 1.90 0.90 2.00 1.60 -0.80 2.00 1.90 0.70 1.00 0.80 0.70 -0.20 2.00 1.10 2.00 2.00 1.80 -0.20 2.00 0.50 -0.10 2.00 1.80 1.40 1.80 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 1.80 1.70 0.80 2.00 2.10 2.00 2.00 1.80 0.80 2.00 1.50 -0.90 2.00 1.80 0.60 0.90 2.30 1.60 1.70 2.00 2.10 1.90 2.00 1.80 1.70 2.00 2.10 0.00 2.00 1.80 1.50 0.90 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.30 1.60 1.70 2.00 2.10 1.90 2.00 1.80 1.70 2.00 2.10 0.00 2.00 1.80 1.50 0.90 0.80 0.70 -0.20 2.00 1.10 2.00 2.00 1.80 -0.20 2.00 0.50 -0.10 2.00 1.80 1.40 1.80 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 1.30 1.20 0.30 2.00 1.70 2.10 2.00 2.00 0.30 2.00 1.10 -0.40 2.00 2.00 1.10 1.50 1.60 0.50 1.40 2.00 0.90 1.80 2.00 1.60 1.40 2.00 1.30 -2.10 2.00 1.60 -0.60 1.60 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.30 1.60 1.70 2.00 2.10 1.90 2.00 1.80 1.70 2.00 2.10 0.00 2.00 1.80 1.50 0.90 0.60 -0.50 0.40 2.00 0.00 0.80 2.00 0.70 0.40 2.00 0.40 -3.10 2.00 0.70 -1.60 0.60 1.90 0.80 1.80 2.00 1.30 1.10 2.00 1.00 1.80 2.00 1.70 0.00 2.00 1.00 1.60 0.10 Y --------------------------------------------------------------------------------------------- A A A A C C C C G G G G U U U U A C G U A C G U A C G U A C G U --------------------------------------------------------------------------------------------- 5' ------> 3' G \/ \_/ U U /\ | A 3' <------ 5' 2.80 2.50 1.50 2.00 2.60 3.10 2.00 3.10 1.50 2.00 2.10 1.30 2.00 3.10 2.30 2.70 2.60 2.40 1.40 2.00 2.50 2.30 2.00 2.30 1.40 2.00 1.90 0.20 2.00 2.30 1.20 1.50 1.50 1.30 0.30 2.00 1.40 2.20 2.00 2.20 0.30 2.00 0.80 0.90 2.00 2.20 1.90 2.20 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.50 2.30 1.30 2.00 2.40 2.20 2.00 2.20 1.30 2.00 1.80 0.10 2.00 2.20 1.10 1.40 3.10 2.20 2.20 2.00 2.30 2.20 2.00 2.20 2.20 2.00 2.40 1.00 2.00 2.20 2.00 1.40 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 3.10 2.20 2.20 2.00 2.30 2.20 2.00 2.20 2.20 2.00 2.40 1.00 2.00 2.20 2.00 1.40 1.50 1.30 0.30 2.00 1.40 2.20 2.00 2.20 0.30 2.00 0.80 0.90 2.00 2.20 1.90 2.20 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.10 1.80 0.80 2.00 1.90 2.40 2.00 2.40 0.80 2.00 1.40 0.70 2.00 2.40 1.60 2.00 2.30 1.10 1.90 2.00 1.20 2.00 2.00 2.00 1.90 2.00 1.60 -1.10 2.00 2.00 -0.10 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 3.10 2.20 2.20 2.00 2.30 2.20 2.00 2.20 2.20 2.00 2.40 1.00 2.00 2.20 2.00 1.40 1.30 0.10 0.90 2.00 0.20 1.00 2.00 1.00 0.90 2.00 0.70 -2.10 2.00 1.00 -1.10 1.10 2.70 1.40 2.20 2.00 1.50 1.40 2.00 1.40 2.20 2.00 2.00 1.10 2.00 1.40 2.00 0.60 Y --------------------------------------------------------------------------------------------- A A A A C C C C G G G G U U U U A C G U A C G U A C G U A C G U --------------------------------------------------------------------------------------------- 5' ------> 3' G \/ \_/ G U /\ | U 3' <------ 5' 2.80 2.30 1.70 2.00 2.80 3.40 2.00 3.40 1.70 2.00 2.10 1.00 2.00 3.10 2.20 2.90 2.60 2.20 1.60 2.00 2.60 2.60 2.00 2.60 1.60 2.00 2.00 -0.20 2.00 2.30 1.10 1.80 1.50 1.10 0.50 2.00 1.50 2.50 2.00 2.50 0.50 2.00 0.90 0.50 2.00 2.20 1.80 2.50 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.50 2.10 1.50 2.00 2.50 2.50 2.00 2.50 1.50 2.00 1.90 -0.30 2.00 2.20 1.00 1.70 3.10 2.00 2.40 2.00 2.50 2.50 2.00 2.50 2.40 2.00 2.40 0.70 2.00 2.20 1.90 1.60 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 3.10 2.00 2.40 2.00 2.50 2.50 2.00 2.50 2.40 2.00 2.40 0.70 2.00 2.20 1.90 1.60 1.50 1.10 0.50 2.00 1.50 2.50 2.00 2.50 0.50 2.00 0.90 0.50 2.00 2.20 1.80 2.50 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.10 1.60 1.00 2.00 2.10 2.70 2.00 2.70 1.00 2.00 1.40 0.30 2.00 2.40 1.50 2.20 2.30 0.90 2.10 2.00 1.30 2.30 2.00 2.30 2.10 2.00 1.70 -1.50 2.00 2.00 -0.20 2.30 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 3.10 2.00 2.40 2.00 2.50 2.50 2.00 2.50 2.40 2.00 2.40 0.70 2.00 2.20 1.90 1.60 1.30 -0.10 1.10 2.00 0.30 1.30 2.00 1.30 1.10 2.00 0.70 -2.50 2.00 1.00 -1.20 1.30 2.70 1.20 2.40 2.00 1.70 1.70 2.00 1.70 2.40 2.00 2.00 0.70 2.00 1.40 1.90 0.80 Y --------------------------------------------------------------------------------------------- A A A A C C C C G G G G U U U U A C G U A C G U A C G U A C G U --------------------------------------------------------------------------------------------- 5' ------> 3' G \/ \_/ U U /\ | G 3' <------ 5' 2.80 2.50 1.50 2.00 2.60 3.10 2.00 3.10 1.50 2.00 2.10 1.30 2.00 3.10 2.30 2.70 2.60 2.40 1.40 2.00 2.50 2.30 2.00 2.30 1.40 2.00 1.90 0.20 2.00 2.30 1.20 1.50 1.50 1.30 0.30 2.00 1.40 2.20 2.00 2.20 0.30 2.00 0.80 0.90 2.00 2.20 1.90 2.20 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.50 2.30 1.30 2.00 2.40 2.20 2.00 2.20 1.30 2.00 1.80 0.10 2.00 2.20 1.10 1.40 3.10 2.20 2.20 2.00 2.30 2.20 2.00 2.20 2.20 2.00 2.40 1.00 2.00 2.20 2.00 1.40 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 3.10 2.20 2.20 2.00 2.30 2.20 2.00 2.20 2.20 2.00 2.40 1.00 2.00 2.20 2.00 1.40 1.50 1.30 0.30 2.00 1.40 2.20 2.00 2.20 0.30 2.00 0.80 0.90 2.00 2.20 1.90 2.20 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.10 1.80 0.80 2.00 1.90 2.40 2.00 2.40 0.80 2.00 1.40 0.70 2.00 2.40 1.60 2.00 2.30 1.10 1.90 2.00 1.20 2.00 2.00 2.00 1.90 2.00 1.60 -1.10 2.00 2.00 -0.10 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 3.10 2.20 2.20 2.00 2.30 2.20 2.00 2.20 2.20 2.00 2.40 1.00 2.00 2.20 2.00 1.40 1.30 0.10 0.90 2.00 0.20 1.00 2.00 1.00 0.90 2.00 0.70 -2.10 2.00 1.00 -1.10 1.10 2.70 1.40 2.20 2.00 1.50 1.40 2.00 1.40 2.20 2.00 2.00 1.10 2.00 1.40 2.00 0.60 Y --------------------------------------------------------------------------------------------- A A A A C C C C G G G G U U U U A C G U A C G U A C G U A C G U --------------------------------------------------------------------------------------------- 5' ------> 3' U \/ \_/ A G /\ | U 3' <------ 5' 2.80 2.30 1.70 2.00 2.80 3.40 2.00 3.40 1.70 2.00 2.10 1.00 2.00 3.10 2.20 2.90 2.80 2.30 1.70 2.00 2.80 2.80 2.00 2.80 1.70 2.00 2.10 0.00 2.00 2.50 1.20 1.90 1.70 1.30 0.70 2.00 1.70 2.70 2.00 2.70 0.70 2.00 1.10 0.70 2.00 2.40 2.00 2.70 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.30 1.90 1.30 2.00 2.30 2.30 2.00 2.30 1.30 2.00 1.70 -0.50 2.00 2.00 0.80 1.50 3.40 2.30 2.70 2.00 2.80 2.80 2.00 2.80 2.70 2.00 2.70 1.00 2.00 2.50 2.20 1.90 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 3.10 2.00 2.40 2.00 2.50 2.50 2.00 2.50 2.40 2.00 2.40 0.70 2.00 2.20 1.90 1.60 1.70 1.30 0.70 2.00 1.70 2.70 2.00 2.70 0.70 2.00 1.10 0.70 2.00 2.40 2.00 2.70 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.10 1.70 1.10 2.00 2.10 2.70 2.00 2.70 1.10 2.00 1.50 0.30 2.00 2.40 1.60 2.30 2.20 0.80 2.00 2.00 1.20 2.20 2.00 2.20 2.00 2.00 1.60 -1.60 2.00 1.90 -0.30 2.20 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 3.40 2.30 2.70 2.00 2.80 2.80 2.00 2.80 2.70 2.00 2.70 1.00 2.00 2.50 2.20 1.90 1.00 -0.50 0.70 2.00 0.00 1.00 2.00 1.00 0.70 2.00 0.30 -2.90 2.00 0.70 -1.60 0.90 2.90 1.50 2.70 2.00 1.90 1.90 2.00 1.90 2.70 2.00 2.30 0.90 2.00 1.60 2.20 1.10 Y --------------------------------------------------------------------------------------------- A A A A C C C C G G G G U U U U A C G U A C G U A C G U A C G U --------------------------------------------------------------------------------------------- 5' ------> 3' U \/ \_/ C G /\ | G 3' <------ 5' 2.10 1.90 0.10 2.00 1.80 2.50 2.00 1.50 0.70 2.00 1.80 0.00 2.00 2.50 0.40 2.10 2.10 1.90 0.10 2.00 1.80 1.90 2.00 0.90 0.70 2.00 1.80 -1.00 2.00 1.90 -0.60 1.10 1.10 0.80 -0.90 2.00 0.80 1.80 2.00 0.90 -0.30 2.00 0.70 -0.30 2.00 1.90 0.10 1.90 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 1.70 1.40 -0.30 2.00 1.40 1.40 2.00 0.40 0.30 2.00 1.30 -1.50 2.00 1.50 -1.10 0.70 2.70 1.90 1.10 2.00 1.80 1.90 2.00 0.90 1.70 2.00 2.40 0.00 2.00 1.90 0.40 1.10 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.40 1.60 0.80 2.00 1.50 1.60 2.00 0.60 1.40 2.00 2.10 -0.30 2.00 1.60 0.10 0.80 1.10 0.80 -0.90 2.00 0.80 1.80 2.00 0.90 -0.30 2.00 0.70 -0.30 2.00 1.90 0.10 1.90 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 1.50 1.20 -0.50 2.00 1.20 1.80 2.00 0.80 0.10 2.00 1.10 -0.70 2.00 1.90 -0.30 1.50 1.60 0.30 0.40 2.00 0.30 1.30 2.00 0.40 1.00 2.00 1.20 -2.60 2.00 1.40 -2.20 1.40 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.70 1.90 1.10 2.00 1.80 1.90 2.00 0.90 1.70 2.00 2.40 0.00 2.00 1.90 0.40 1.10 0.30 -0.90 -0.90 2.00 -1.00 0.10 2.00 -0.90 -0.30 2.00 0.00 -3.90 2.00 0.10 -3.50 0.10 2.30 1.00 1.10 2.00 1.00 1.00 2.00 0.00 1.70 2.00 1.90 -0.10 2.00 1.10 0.30 0.30 Y --------------------------------------------------------------------------------------------- A A A A C C C C G G G G U U U U A C G U A C G U A C G U A C G U --------------------------------------------------------------------------------------------- 5' ------> 3' U \/ \_/ G G /\ | C 3' <------ 5' 2.00 1.90 1.00 2.00 2.40 2.80 2.00 2.70 1.00 2.00 1.80 0.30 2.00 2.70 1.80 2.20 2.00 1.90 1.00 2.00 2.40 2.20 2.00 2.10 1.00 2.00 1.80 -0.70 2.00 2.10 0.80 1.20 1.00 0.90 0.00 2.00 1.30 2.20 2.00 2.00 0.00 2.00 0.70 0.10 2.00 2.00 1.60 1.90 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 1.60 1.50 0.60 2.00 1.90 1.80 2.00 1.60 0.60 2.00 1.30 -1.10 2.00 1.60 0.40 0.70 2.60 1.90 2.00 2.00 2.40 2.20 2.00 2.10 2.00 2.00 2.40 0.30 2.00 2.10 1.80 1.20 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.30 1.60 1.70 2.00 2.10 1.90 2.00 1.80 1.70 2.00 2.10 0.00 2.00 1.80 1.50 0.90 1.00 0.90 0.00 2.00 1.30 2.20 2.00 2.00 0.00 2.00 0.70 0.10 2.00 2.00 1.60 1.90 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 1.40 1.30 0.40 2.00 1.70 2.20 2.00 2.00 0.40 2.00 1.10 -0.30 2.00 2.00 1.20 1.50 1.50 0.40 1.30 2.00 0.80 1.70 2.00 1.50 1.30 2.00 1.20 -2.20 2.00 1.50 -0.70 1.50 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.60 1.90 2.00 2.00 2.40 2.20 2.00 2.10 2.00 2.00 2.40 0.30 2.00 2.10 1.80 1.20 0.20 -0.90 0.00 2.00 -0.40 0.40 2.00 0.30 0.00 2.00 0.00 -3.50 2.00 0.30 -2.00 0.20 2.20 1.10 2.00 2.00 1.50 1.40 2.00 1.20 2.00 2.00 1.90 0.30 2.00 1.20 1.80 0.30 Y --------------------------------------------------------------------------------------------- A A A A C C C C G G G G U U U U A C G U A C G U A C G U A C G U --------------------------------------------------------------------------------------------- 5' ------> 3' U \/ \_/ U G /\ | A 3' <------ 5' 2.80 2.50 1.50 2.00 2.60 3.10 2.00 3.10 1.50 2.00 2.10 1.30 2.00 3.10 2.30 2.70 2.80 2.50 1.50 2.00 2.60 2.50 2.00 2.50 1.50 2.00 2.10 0.30 2.00 2.50 1.30 1.70 1.70 1.50 0.50 2.00 1.60 2.40 2.00 2.40 0.50 2.00 1.00 1.10 2.00 2.40 2.10 2.40 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.30 2.10 1.10 2.00 2.20 2.00 2.00 2.00 1.10 2.00 1.60 -0.10 2.00 2.00 0.90 1.20 3.40 2.50 2.50 2.00 2.60 2.50 2.00 2.50 2.50 2.00 2.70 1.30 2.00 2.50 2.30 1.70 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 3.10 2.20 2.20 2.00 2.30 2.20 2.00 2.20 2.20 2.00 2.40 1.00 2.00 2.20 2.00 1.40 1.70 1.50 0.50 2.00 1.60 2.40 2.00 2.40 0.50 2.00 1.00 1.10 2.00 2.40 2.10 2.40 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.10 1.90 0.90 2.00 2.00 2.40 2.00 2.40 0.90 2.00 1.40 0.70 2.00 2.40 1.70 2.00 2.20 1.00 1.80 2.00 1.10 1.90 2.00 1.90 1.80 2.00 1.50 -1.20 2.00 1.90 -0.20 1.90 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 3.40 2.50 2.50 2.00 2.60 2.50 2.00 2.50 2.50 2.00 2.70 1.30 2.00 2.50 2.30 1.70 1.00 -0.30 0.50 2.00 -0.20 0.70 2.00 0.70 0.50 2.00 0.30 -2.50 2.00 0.70 -1.50 0.70 2.90 1.70 2.50 2.00 1.80 1.60 2.00 1.60 2.50 2.00 2.20 1.30 2.00 1.60 2.30 0.80 Y --------------------------------------------------------------------------------------------- A A A A C C C C G G G G U U U U A C G U A C G U A C G U A C G U --------------------------------------------------------------------------------------------- 5' ------> 3' U \/ \_/ G G /\ | U 3' <------ 5' 2.80 2.30 1.70 2.00 2.80 3.40 2.00 3.40 1.70 2.00 2.10 1.00 2.00 3.10 2.20 2.90 2.80 2.30 1.70 2.00 2.80 2.80 2.00 2.80 1.70 2.00 2.10 0.00 2.00 2.50 1.20 1.90 1.70 1.30 0.70 2.00 1.70 2.70 2.00 2.70 0.70 2.00 1.10 0.70 2.00 2.40 2.00 2.70 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.30 1.90 1.30 2.00 2.30 2.30 2.00 2.30 1.30 2.00 1.70 -0.50 2.00 2.00 0.80 1.50 3.40 2.30 2.70 2.00 2.80 2.80 2.00 2.80 2.70 2.00 2.70 1.00 2.00 2.50 2.20 1.90 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 3.10 2.00 2.40 2.00 2.50 2.50 2.00 2.50 2.40 2.00 2.40 0.70 2.00 2.20 1.90 1.60 1.70 1.30 0.70 2.00 1.70 2.70 2.00 2.70 0.70 2.00 1.10 0.70 2.00 2.40 2.00 2.70 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.10 1.70 1.10 2.00 2.10 2.70 2.00 2.70 1.10 2.00 1.50 0.30 2.00 2.40 1.60 2.30 2.20 0.80 2.00 2.00 1.20 2.20 2.00 2.20 2.00 2.00 1.60 -1.60 2.00 1.90 -0.30 2.20 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 3.40 2.30 2.70 2.00 2.80 2.80 2.00 2.80 2.70 2.00 2.70 1.00 2.00 2.50 2.20 1.90 1.00 -0.50 0.70 2.00 0.00 1.00 2.00 1.00 0.70 2.00 0.30 -2.90 2.00 0.70 -1.60 0.90 2.90 1.50 2.70 2.00 1.90 1.90 2.00 1.90 2.70 2.00 2.30 0.90 2.00 1.60 2.20 1.10 Y --------------------------------------------------------------------------------------------- A A A A C C C C G G G G U U U U A C G U A C G U A C G U A C G U --------------------------------------------------------------------------------------------- 5' ------> 3' U \/ \_/ U G /\ | G 3' <------ 5' 2.80 2.50 1.50 2.00 2.60 3.10 2.00 3.10 1.50 2.00 2.10 1.30 2.00 3.10 2.30 2.70 2.80 2.50 1.50 2.00 2.60 2.50 2.00 2.50 1.50 2.00 2.10 0.30 2.00 2.50 1.30 1.70 1.70 1.50 0.50 2.00 1.60 2.40 2.00 2.40 0.50 2.00 1.00 1.10 2.00 2.40 2.10 2.40 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.30 2.10 1.10 2.00 2.20 2.00 2.00 2.00 1.10 2.00 1.60 -0.10 2.00 2.00 0.90 1.20 3.40 2.50 2.50 2.00 2.60 2.50 2.00 2.50 2.50 2.00 2.70 1.30 2.00 2.50 2.30 1.70 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 3.10 2.20 2.20 2.00 2.30 2.20 2.00 2.20 2.20 2.00 2.40 1.00 2.00 2.20 2.00 1.40 1.70 1.50 0.50 2.00 1.60 2.40 2.00 2.40 0.50 2.00 1.00 1.10 2.00 2.40 2.10 2.40 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.10 1.90 0.90 2.00 2.00 2.40 2.00 2.40 0.90 2.00 1.40 0.70 2.00 2.40 1.70 2.00 2.20 1.00 1.80 2.00 1.10 1.90 2.00 1.90 1.80 2.00 1.50 -1.20 2.00 1.90 -0.20 1.90 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 3.40 2.50 2.50 2.00 2.60 2.50 2.00 2.50 2.50 2.00 2.70 1.30 2.00 2.50 2.30 1.70 1.00 -0.30 0.50 2.00 -0.20 0.70 2.00 0.70 0.50 2.00 0.30 -2.50 2.00 0.70 -1.50 0.70 2.90 1.70 2.50 2.00 1.80 1.60 2.00 1.60 2.50 2.00 2.20 1.30 2.00 1.60 2.30 0.80

Single base stacking energies X X X X ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' AX AX AX AX A C G U 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' . . . . . . . . . . . . -0.80 -0.50 -0.80 -0.60 X X X X ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' CX CX CX CX A C G U 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' . . . . . . . . -1.70 -0.80 -1.70 -1.20 . . . . X X X X ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' GX GX GX GX A C G U 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' . . . . -1.10 -0.40 -1.30 -0.60 . . . . -0.80 -0.50 -0.80 -0.60 X X X X ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' UX UX UX UX A C G U 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' -0.70 -0.10 -0.70 -0.10 . . . . -0.70 -0.10 -0.70 -0.10 . . . . X X X X ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' A A A A AX CX GX UX 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' . . . . . . . . . . . . -0.30 -0.10 -0.20 -0.20 X X X X ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' C C C C AX CX GX UX 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' . . . . . . . . -0.20 -0.30 -0.00 -0.00 . . . . X X X X ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' G G G G AX CX GX UX 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' . . . . -0.50 -0.30 -0.20 -0.10 . . . . -0.30 -0.10 -0.20 -0.20 X X X X ------------------ ------------------ ------------------ ------------------ A C G U A C G U A C G U A C G U ------------------ ------------------ ------------------ ------------------ 5' --> 3' 5' --> 3' 5' --> 3' 5' --> 3' U U U U AX CX GX UX 3' <-- 5' 3' <-- 5' 3' <-- 5' 3' <-- 5' -0.30 -0.30 -0.40 -0.20 . . . . -0.30 -0.30 -0.40 -0.20 . . . .

Loop destabilizing energies DESTABILIZING ENERGIES BY SIZE OF LOOP (INTERPOLATE WHERE NEEDED) hp3 ave calc no tmm;hp4 ave calc with tmm; ave all bulges SIZE INTERNAL BULGE HAIRPIN ------------------------------------------------------- 1 . 3.80 . 2 . 2.80 . 3 . 3.20 5.70 4 1.70 3.60 5.60 5 1.80 4.00 5.60 6 2.00 4.40 5.40 7 2.20 4.60 5.90 8 2.30 4.70 5.60 9 2.40 4.80 6.40 10 2.50 4.90 6.50 11 2.60 5.00 6.60 12 2.70 5.10 6.70 13 2.80 5.20 6.80 14 2.90 5.30 6.90 15 3.00 5.40 6.90 16 3.00 5.40 7.00 17 3.10 5.50 7.10 18 3.10 5.50 7.10 19 3.20 5.60 7.20 20 3.30 5.70 7.20 21 3.30 5.70 7.30 22 3.40 5.80 7.30 23 3.40 5.80 7.40 24 3.40 5.80 7.40 25 3.50 5.90 7.50 26 3.50 5.90 7.50 27 3.60 6.00 7.50 28 3.60 6.00 7.60 29 3.60 6.00 7.60 30 3.70 6.10 7.70

Tetra-loops Seq Energy ------------- GGGGAC -3.00 GGUGAC -3.00 CGAAAG -3.00 GGAGAC -3.00 CGCAAG -3.00 GGAAAC -3.00 CGGAAG -3.00 CUUCGG -3.00 CGUGAG -3.00 CGAAGG -2.50 CUACGG -2.50 GGCAAC -2.50 CGCGAG -2.50 UGAGAG -2.50 CGAGAG -2.00 AGAAAU -2.00 CGUAAG -2.00 CUAACG -2.00 UGAAAG -2.00 GGAAGC -1.50 GGGAAC -1.50 UGAAAA -1.50 AGCAAU -1.50 AGUAAU -1.50 CGGGAG -1.50 AGUGAU -1.50 GGCGAC -1.50 GGGAGC -1.50 GUGAAC -1.50 UGGAAA -1.50

Tri-loops Seq Energy -------------

Miscellaneous energies Miscellaneous free energy rules ------------------------------- Extrapolation for large loops based on polymer theory internal, bulge or hairpin loops > 30: dS(T)=dS(30)+param*ln(n/30) --> 1.079 asymmetric internal loops: the ninio equation the maximum correction --> 3.00 the f(m) array (see Ninio for details) --> .50 .50 .50 .50 multibranched loops offset, free base penalty, helix penalty --> 3.40 .00 .40 efn2 multibranched loops offset, free base penalty, helix penalty --> 10.10 -0.30 -0.30 terminal AU penalty --> 0.50 bonus for GGG hairpin --> -2.20 c hairpin slope --> 0.30 c hairpin intercept --> 1.60 c hairpin of 3 --> 1.40 Intermolecular initiation free energy --> 4.10 GAIL Rule (Grossly Asymmetric Interior Loop Rule) (on/off <-> 1/0) --> 1