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We have found, using electron tomography, that the cristae
are actually pleomorphic compartments, with narrow tubular connections
to the peripheral membrane surface and to each other (>> view
images ). Computer modeling (in
collaboration with Moraru et al, Univ. Connecticut) indicates that
the shape of the tubular junctions can regulate ATP production by
restricting the lateral diffusion of molecules. Clues about the molecular
factors that control IM shape are being provided by collaborative
studies using model systems. In mouse liver mitochondria, alterations
in IM morphology are induced by a protein (tBID) that triggers cytochrome
c release during apoptosis (Scorrano et al, Harvard Univ). In the
nematode, C. elegans, mutation of the dynamin-like protein, mgm-1,
results in highly aberrant IM topology (Van der Bliek et al, UCLA).
In humans, mutation of the same mitochondrial protein is associated
with a hereditary disorder, dominant optic atrophy. (read
more about research of Dr. Mannella).
Main publications in Mitochondrial Structure:
• Munn, E.A. (1974) The
Structure of Mitochondria. Academic Press, London.
Online resources in Mitochondrial Structure:
• www.cytochemistry.net
Dr. Mannella's publications in Mitochondrial Structure:
•
Journal articles:
Mannella CA, Pfeiffer DR, Bradshaw PC, Moraru II, Slepchenko B, Loew
LM, Hsieh CE, Buttle K, Marko M. Topology of the mitochondrial inner
membrane: dynamics and bioenergetic implications. IUBMB Life. 2001
Sep-Nov;52(3-5):93-100.
Verschoor, A., Tivol, W.F., Mannella, C.A. (2001) J. Struct. Biol.
133, 254-265. Single-particle approaches in the analysis of small
2D crystals of the mitochondrial channel, VDAC.
Frey, T, Mannella,
C.A. (2000) Trends Biochem. Sci. 25, 319-324. The internal structure
of mitochondria [with cover].
Deng, Y., Marko,
M., Buttle, K.F., Leith, A., Mieczkowski, M., Mannella, C.A. (1999)
J. Struct. Biol. 127, 231-239. Cubic membrane structure in amoeba
(Chaos carolinensis) mitochondria determined by electron microscopic
tomography [with cover].
Mannella, C.A.,
Neuwald, A.F., Lawrence, C.E. (1996) J. Bioenerg. Biomembr. 28,
163-169. Detection of likely transmembrane b-strand regions in
sequences of mitochondrial pore proteins using the Gibbs sampler.
Guo, X.W.,
Mannella, C.A. (1992) Biophys. J. 63, 418-427. Classification of
projection images of crystalline arrays of the mitochondrial channel,
VDAC, embedded in aurothioglucose.
Forte, M.,
Guy, H.R., Mannella, C.A. (1987) J. Bioenerg. Biomembr. 19, 341-350.
Molecular genetics of the VDAC ion channel: structural model and
sequence analysis.
>> more publications
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